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Cto 6a

Manufactured by Shimadzu
Sourced in Japan

The CTO-6A is a column thermostat designed for HPLC systems. It provides precise temperature control of the column compartment to ensure the stability and reproducibility of chromatographic separations.

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2 protocols using cto 6a

1

Quantifying Free Amino Acids by HPLC

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Free amino acids were estimated by a high-performance liquid chromatographic (HPLC) system (Shimadzu Corporation, Kyoto, Japan) consisting of a system controller (SCL-6B), an auto-injector (SIL-6B), HPLC pumps (LC-6A), column oven (CTO-6A), fluorescence detector (RF-551), and a Supelcosil LC-18 column (5 mm packing, 150 × 4.6 mm). Briefly, 100 mg of sliced biological replicates was chosen randomly from each plot and dried to a constant weight for 16 h at 80 °C. The extraction was measured to determine free amino acids according to the method of Hansen [53 (link)]
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2

HPLC Analysis of Compounds

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The HPLC system consisted of a Shimadzu LC-10 AD pump (Kyoto, Japan) equipped with a Shimadzu RF-5300 fluorescence detector and a Shimadzu CTO-6A column oven. The analytical column was a reverse-phase Hydrosphere C18 column (internal diameter [i.d.], 4.6 × 50 mm, 5 μm), which was purchased from YMC Co., Ltd. (Kyoto, Japan).
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