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Facs calibur e6147 cytometer

Manufactured by BD
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The FACS-Calibur E6147 Cytometer is a flow cytometry instrument designed for analyzing and sorting cells. It is capable of detecting and measuring various cellular properties, including size, granularity, and fluorescence, using laser-based technology. The FACS-Calibur E6147 Cytometer is a compact and versatile instrument suitable for a range of applications in research and clinical settings.

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Lab products found in correlation

Annexin 5 fitc, by Abcam (2 mentions) Cellquest pro 4, by BD (1 mentions)

Close spelling variants of this product

Calibur (250 citations) Calibur cytometer (14 citations) BD FACSTM (2 citations)

3 protocols using facs calibur e6147 cytometer

1

Cell Death Induction and Analysis

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Cells were treated with 125 μM of H2O2 for 16 hours or serum starvation for 5 days to induce cell death. Cell cycle of sub-G1 analysis was performed and quantified using the FACS-Calibur E6147 Cytometer and CellQuest Pro 4.02 software (BD Biosciences) as described previously [21 (link)].
Chiang K.C., Tsui K.H., Lin Y.H., Hou C.P., Chang K.S., Tsai H.H., Shin Y.S., Chen C.C., Feng T.H, & Juang H.H. (2019). Antioxidation and Antiapoptosis Characteristics of Heme Oxygenase-1 Enhance Tumorigenesis of Human Prostate Carcinoma Cells. Translational Oncology, 13(1), 102-112.
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2

Quantification of Apoptosis After H2O2 Treatment

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The cell pellets were harvested after treated with H2O2 (500 μM) for 12 hours. The detection and quantification of cell apoptosis were performed after treated with Annexin V-FITC (BioVision Inc, Milpitas, CA) using the FACS-Calibur E6147 Cytometer (BD Biosciences) as described previously [22 ].
Chiang K.C., Tsui K.H., Lin Y.H., Hou C.P., Chang K.S., Tsai H.H., Shin Y.S., Chen C.C., Feng T.H, & Juang H.H. (2019). Antioxidation and Antiapoptosis Characteristics of Heme Oxygenase-1 Enhance Tumorigenesis of Human Prostate Carcinoma Cells. Translational Oncology, 13(1), 102-112.
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3

Apoptosis Detection by Flow Cytometry

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Cell pellets were harvested after cells were treated with or without arsenic (AS2O3) for 24 h. Apoptosis detection and quantification were performed after treatments with Annexin V-FITC (BioVision Inc, Milpitas, CA, USA) and propidium iodide (PI) for 1 h using the FACSCalibur E6147 Cytometer (BD Biosciences) as described previously [51 (link)].
Tsui K.H., Hou C.P., Chang K.S., Lin Y.H., Feng T.H., Chen C.C., Shin Y.S, & Juang H.H. (2019). Metallothionein 3 Is a Hypoxia-Upregulated Oncogene Enhancing Cell Invasion and Tumorigenesis in Human Bladder Carcinoma Cells. International Journal of Molecular Sciences, 20(4), 980.
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