Rat anti mouse igg2a hrp

Antibodies were obtained from the following commercial sources: anti-tubulin (Sigma, St. Louis, MO), anti-GLUT1 (Cell Signaling, Beverly, MA), anti-HIF-1ɑ and anti-HIF-2ɑ (Novus Biologicals, Littleton, CO), anti-CXCL10 (Abcam, Cambridge, MA for IHC; R&D Systems, Minneapolis, MN for neutralization studies), anti-CD31 (BD Biosciences, San Jose, CA), rat anti-mouse IgG2a-HRP (Serotec, Raleigh, NC), and sheep anti-mouse-HRP and donkey anti-rabbit-HRP (Amersham, Pittsburgh, PA). Anti-reovirus antibody and Reolysin were kindly provided by Oncolytics Biotech, Inc. (Calgary, AB, Canada). Alexa Fluor 488 rabbit anti-goat was obtained from Molecular Probes (Eugene, OR). Sunitinib, bevacizumab, and temsirolimus were purchased from the hospital pharmacy.

Antibodies were obtained from the following commercial sources: p-JNK (#4668), JNK (#9252), caspase-8 (#9746), BCL-2 (#4223), c-IAP-1 (#4952), c-IAP-2 (#3130), survivin (#2808), cleaved caspase-3 (#9661), FLIP (#56343), and XIAP (#2045) (Cell Signaling Technology, Danvers, MA, USA), β–Actin (sc-58673) (Santa Cruz Technologies, Santa Cruz, CA, USA), and tubulin (T7816) (Sigma, St. Louis, MO, USA). For detection, appropriate goat anti-rabbit and goat anti-rat horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson Laboratories, West Grove, PA, USA), rat anti-mouse IgG2a-HRP (Serotec, Raleigh, NC, USA), and sheep anti-mouse-HRP and donkey anti-rabbit-HRP (Amersham, Pittsburgh, PA, USA) were used. Sunitinib was purchased from the hospital pharmacy. To induce apoptosis, cells were treated with rhTRAIL (non-tagged, fragment of amino acids 114-281, Triskel Therapeutics, Groningen, The Netherlands).