Libra 200 ht fe mc

Overnight cultures grown in SMM were concentrated via centrifugation for 30 min at 4,000 rpm, washed with 10 mM Tris buffer (pH 8.0), and centrifuged again for 2 min at 4,000 rpm prior to negative staining (79 (link)). Pelleted cells were placed on ice and fixed for 1 h in a 3% (vol/vol) glutaraldehyde solution. Aliquots (10 μL) were added to a 200-mesh Formvar/carbon-coated copper grid and adsorbed for 3 min at ambient temperature followed by staining for 1 min with 0.5% (wt/vol) aqueous uranyl acetate. Samples were examined on a Zeiss Libra 200 HT FE MC transmission electron microscope using an acceleration voltage of 80 kV.

The studied alloys were characterized before and after LCF tests, using SEM, EBSD, EDS, and TEM techniques. The microstructure was characterized, by SEM, employing a Zeiss EVO scanning-electron microscope equipped with the backscattering electron (BSE), EDS, and EBSD detectors. The crystal structures of this multiphase alloy were identified by HEXRD with a wavelength of 0.117418 Å on the 11-ID-C beam line at the Advanced Photon Source (APS), Argonne National Laboratory. The specimens for SEM and EBSD were initially polished to mirror finish, using the 1200-grit SiC paper and, subsequently, vibratorily polished, employing the 0.05-μm SiC suspension for the final surface clarification. The TEM was performed on a ZEISS LIBRA 200 HT FE MC. Thin foils for the TEM observations were electrical-discharge machined (EDM) from the gauge section of ruptured samples. The thin foils converted into 3-mm-diameter disks for the TEM observations were prepared by twin-jet polishing, using an electrolyte consisting of 95% (volume percent) ethanol and 5% perchloric acid in a volume fraction at a temperature of −40 °C and an applied voltage of 30 V. The examination of many specimens, including foils from the undeformed section, exhibited that very few artifact dislocations were produced by this TEM sample-preparation technique.

Microscopy was performed by the Advanced Microscopy and Imaging Center, at the Joint Institute for Advanced Materials (University of Tennessee, Knoxville). High titer bacteriophage particles were isolated by PEG precipitation, stained with potassium phosphotungstic acid (KPTA) or uranyl acetate (UAc), respectively, and imaged using a Zeiss Libra 200 HT FE MC transmission electron microscope.