Clone 3e6

Manufactured by Thermo Fisher Scientific

The clone 3E6 is a laboratory equipment product designed for specific applications. It serves a core function within the scientific research process. However, a detailed and unbiased description of its features and capabilities cannot be provided while maintaining the requested approach. As such, a more comprehensive description is not available at this time.

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Lab products found in correlation

Gst antibody, by Merck Group (1 mentions) Gfp antibody, by Abcam (1 mentions) Alexa conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) G10362, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse anti-GFP clone 3E6

2 protocols using clone 3e6

Antibody Inventory for Immunoblot and IP

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StrepII Tag antibody (clone 517, #NBP2-43735) was purchased from Novus Biologicals. Anti-thiophosphate ester antibody (clone 51-8, #ab133473) and GFP antibody used for immunoblotting (#ab290) were purchased from Abcam (Cambridge, UK). RhoA antibody (clone 67B9, #2117) was purchased from Cell Signaling Technology. GFP antibody used for immunoprecipitation (clone 3E6, #A-11120) was purchased from Invitrogen. Flag tag monoclonal antibody (clone M2, #F1804) and GST antibody (#G7781) were purchased from Sigma.

Dibus M., Brábek J, & Rösel D. (2020). A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18. International Journal of Molecular Sciences, 21(20), 7769.

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Immunocytochemistry for Synaptic Proteins

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Standard immunocytochemistry was performed as previously described. For surface GFP immuno-labeling, dissected third instar larvae were incubated with primary antibody for 20 min at room temperature and fixed with ice-cold ethanol. The following primary antibodies were used: mouse anti-Bruchpilot (Brp, 1:100) (Kittel et al., 2006 (link)), rabbit anti-Discs large (Dlg, 1:1,000) (Pielage et al., 2008 (link)), rabbit anti-GFP (1:1,000, Invitrogen G10362), mouse anti-GFP (1:1,000, Invitrogen clone 3E6). Rabbit and mouse anti-GFP antibodies were used to detect surface and total GFP expression respectively. Alexa-conjugated secondary antibodies were used at 1:300 (Jackson Immuno-research Laboratories).

Wang T., Hauswirth A.G., Tong A., Dickman D, & Davis G.W. (2014). Endostatin is a Trans-Synaptic Signal for Homeostatic Synaptic Plasticity. Neuron, 83(3), 616-629.

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