Mouse anti nanog

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse anti-Nanog is an antibody that specifically binds to the Nanog protein, which is a transcription factor essential for the self-renewal and pluripotency of embryonic stem cells. This antibody can be used to detect and study the expression and localization of Nanog in various cell and tissue types.

Automatically generated - may contain errors

Lab products found in correlation

Ab97959, by Santa Cruz Biotechnology (1 mentions) Mouse anti sox2, by Merck Group (1 mentions) Mouse anti ha 11 clone 16b12 monoclonal, by Fortrea (1 mentions) Inverted fluorescence microscope, by Nikon (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Ap detection kit, by Merck Group (1 mentions) Diaminophenylindole dapi, by Merck Group (1 mentions) Goat anti gata4, by Santa Cruz Biotechnology (1 mentions) Mouse anti sox2, by Abcam (1 mentions) Goat anti oct4, by Abcam (1 mentions) Hrp conjugated goat anti rabbit antibody, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse hrp conjugated antibody, by Santa Cruz Biotechnology (1 mentions) Mouse anti vimentin, by Santa Cruz Biotechnology (1 mentions) Egfr antibody, by Santa Cruz Biotechnology (1 mentions) Mouse anti cd44, by Cell Signaling Technology (1 mentions) Rabbit anti n cadherin, by Abcam (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Western blotting luminol reagent, by Bio-Rad (1 mentions) Mouse anti sox2, by R&D Systems (1 mentions) Anti e cadherin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Nanog (81 citations) Anti-Nanog (37 citations)

6 protocols using mouse anti nanog

Proximity Mapping of Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

To identify proximity between various combinations of transcription factors, we performed proximity ligation assays (PLAs) as previously described (Alam, 2018 (link)) using the DuoLink Fluorescence approach (Sigma-Aldrich, DUO92101) with minor modifications. For PLA in chick embryo sections, embryos were fixed with phosphate buffer (PB) containing 4% PFA for 20 minutes and cryosectioned in OCT compound. Primary antibody pairs used are as follows: goat anti-SOX2 (R&D Systems AF2018), rabbit anti-OCT4 (Invitrogen 701756). For PLA in human cells, we fixed cells at day 0 and day 5 of neural crest induction in 4% PFA for 10 minutes at room temperature. We then permeabilized the cells with 0.1% NP-40 in PBS for 30 minutes and 37°C, followed by blocking and primary antibody incubation in 1% BSA in PBS overnight at 4°C. Primary antibody pairs used are as follows: rabbit anti-SOX2 (Abcam ab97959), mouse anti-NANOG (Santa Cruz Biotechnology sc-293121); rabbit anti-OCT4 (Abcam ab109183), mouse anti-NANOG (Santa Cruz Biotechnology sc-293121); rabbit anti-SOX2 (Abcam ab97959), mouse anti-Tfap2a (DSHB 3B5); rabbit anti-Oct4 (Abcam ab109183), mouse anti-TFAP2A (DSHB 3B5)

Hovland A.S., Bhattacharya D., Azambuja A.P., Pramio D., Copeland J., Rothstein M, & Simoes-Costa M. (2022). Pluripotency factors are repurposed to shape the epigenomic landscape of neural crest cells. Developmental cell, 57(19), 2257-2272.e5.

+ Open protocol

+ Expand

Immunostaining of Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 4% paraformaldehyde (PFA) for 15 min. The fixative solution was removed and the cells were washed with PBS 3 times for 5 min each. Cells were then incubated with a blocking buffer consisting of 0.20% Triton X-100, 3 mM sodium azide, 0.1% saponin, 2% BSA, and 5% donkey serum in PBS for 30 min to 1 h at room temperature. Cells were then incubated with primary antibody (mouse anti-HA.11 clone 16B12 monoclonal 1:1,000; Covance, mouse anti-Sox2 1:250; Millipore, rabbit Oct3/4 1:500, Santa Cruz Biotechnology, mouse anti-Nanog 1:400, Santa Cruz Biotechnology) overnight at 4ºC. After 3 washes with PBS, cells were incubated with secondary antibody (anti-mouse Cy-5 conjugated 1:1,000; Jackson ImmunoResearch) for 30-45 min at room temperature. Hoechst staining was performed (0.12 µg/ml) to visualize the nucleus.

Cho I.K., Moran S.P., Paudyal R., Piotrowska-Nitsche K., Cheng P.H., Zhang X., Mao H, & Chan A.W. (2014). Longitudinal Monitoring of Stem Cell Grafts In Vivo Using Magnetic Resonance Imaging with Inducible Maga as a Genetic Reporter. Theranostics, 4(10), 972-989.

+ Open protocol

+ Expand

Immunophenotyping Stem Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostaining assay was performed according to standard protocols. Briefly, the cells were fixed in 4% paraformaldehyde and washed with phosphate-buffered saline (PBS) (Invitrogen, 10010023) and then incubated in PBS containing 0.2% Triton X-100 (Sigma-Aldrich, 9002931) and 0.3% bovine serum albumin (Invitrogen, 11020021). Afterwards, the cells were incubated with primary antibodies including: goat anti-Oct4 (Abcam, ab27985), mouse anti-Nanog (Santa Cruz, sc-374001), mouse anti-Sox2 (Abcam, ab75485), goat anti-GATA4 (Santa Cruz, sc-1237), mouse anti-tyrosine hydroxylase (TH) (Abcam, ab6211), and goat anti- alpha fetoprotein (AFP) (Santa Cruz, sc-8108). The cells were then washed and incubated with goat anti-mouse IgG or rabbit-anti-goat IgG (Invitrogen). A concentration of 0.5 µg/ml diamino phenyl indole (DAPI) (Sigma, 28718903) was used for nuclei staining. Images were visualized using Nikon Ti inverted fluorescence microscope. Alkaline phosphatase (AP) staining was performed using the AP detection kit (Millipore, SCR004).

Peng X., Liu T., Shi C., Zhang L., Wang Y., Zhao W., Jiang L., Wu M., Zhang Y, & Qian Q. (2014). Germline Transmission of an Embryonic Stem Cell Line Derived from BALB/c Cataract Mice. PLoS ONE, 9(3), e90707.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aliquots of protein (30 µg) were subjected to Western blotting. The membranes were incubated with a rabbit polyclonal EGFR antibody (Santa Cruz Biotechnology, USA), a mouse anti-vimentin (Santa Cruz Biotechnology, USA), a mouse anti-NANOG (Santa Cruz Biotechnology, USA), a mouse anti-Sox2 (R&D Systems, USA), a rabbit anti-N-cadherin (Abcam, USA), a rabbit anti-fibronectin 1 (Merck, USA), a mouse anti-CD44 (Cell Signaling, USA) and anti-E-cadherin (Santa Cruz Biotechnology, USA). After washing with TBS-Tween (Merck, USA), the membranes were incubated with HRP-conjugated goat anti-rabbit antibody (Santa Cruz Biotechnology, USA) or HRP-conjugated goat-anti-mouse antibody (Santa Cruz Biotechnology, USA). Equal loading was verified by reprobing the membranes with HRP-conjugated anti-GAPDH antibody (Novus Biologicals, USA). The bands were visualized with Western Blotting Luminol Reagent (Bio Rad, USA) using Chemi Doc™ MP Imaging System (Bio Rad, USA).

Melissaridou S., Wiechec E., Magan M., Jain M.V., Chung M.K., Farnebo L, & Roberg K. (2019). The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer. Cancer Cell International, 19, 16.

+ Open protocol

+ Expand

Immunocytochemical Characterization of iPSC-Derived Motor Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured human iPSCs or stem cell-derived motor neurons were fixed for 10 min in 4% PFA, then permeabilized and blocked for 1 hr in 1% goat serum and 0.1% Triton X-100 in PBS. Primary antibodies were applied overnight at 4°C in Shandon coverplates. Primary antibodies used for stem cell characterization include mouse anti-TRA-1–81 (Millipore, 1:200), rabbit anti-Oct4 (ThermoFisher, 1:1,000), goat anti-LIN-28A (R&D Biosystems, 1:50), mouse anti-Nanog (Santa Cruz, 1:200), rabbit anti-α fetoprotein (Dako, 1:400), mouse anti-α smooth muscle Actin (Sigma, 1:400), mouse anti-Nestin (ThermoFisher, 1:200), rabbit anti-PAX6 (BioLegend, 1:2000).
Primary antibodies used for motor neuron characterization include mouse anti-Islet-1/2 (DSHB, 1:500), mouse anti-HB9/Mnx1 (DSHB, 1:500), goat anti-SCIP (Santa Cruz, 1:5000), anti-HOXA5 (Dasen et al., 2005 (link)), anti-FOXP1 (Dasen et al., 2008 (link)), anti-HOXC6 (Liu et al., 2001 (link)), anti-HOXC9 (Jung et al., 2010 (link)), anti-LHX3 (Tsuchida et al., 1994 (link)), rabbit anti-OLIG2 (Proteintech, 1:500), mouse anti-p-Histone H3 (Santa Cruz, 1:400), Alexa 488 pre-conjugated rabbit anti-Cleaved Caspase-3 (Cell Signaling, 1:50). For detection, we used Alexa fluor [488, 555, 594, 647]-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (Invitrogen, 1:1,000).

LaForce G.R., Farr J.S., Liu J., Akesson C., Gumus E., Pinkard O., Miranda H.C., Johnson K., Sweet T.J., Ji P., Lin A., Coller J., Philippidou P., Wagner E.J, & Schaffer A.E. (2022). Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron, 110(8), 1340-1357.e7.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultured cells were fixed in 4% paraformaldehyde (PFA) (Sigma Aldrich) in PBS for 10 min and then permeabilized in PBS containing 0.3% Triton X-100 (Sigma Aldrich). Cultures were then incubated with primary antibodies followed by secondary antibodies (see dilutions below). 4',6-diamidino-2-phenylindole (DAPI) (1:1000) (Life Technologies) was added to visualize cell nuclei. Images were taken with an inverted fluorescence microscope and attached camera (Nikon Eclipse Ti). Primary antibodies used in the study were: anti-HA-tag (Cat# ab94918, Abcam, 1:200), mouse anti-Nanog (Cat# sc-134218, Santa Cruz, 1:100). Secondary antibodies used in the study (all 1:400) were AF488 donkey anti-goat (Cat# A32814, Life Technologies), AF555 donkey anti-mouse IgG (Cat# A31570, Life Technologies).

Knaupp A.S., Mohenska M., Larcombe M.R., Ford E., Lim S., Wong K.W., Chen J., Firas J., Huang C., Liu X., Nguyen T.A., Sun Y., Holmes M., Tripathi P., Rossello F.J., Schröder J., Nefzger C.M., Das P., Haigh J., Lister R., Schittenhelm R.B., & Polo J.M. (2020). TINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!