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Stempro osteogenesis differentiation kit medium

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom

The Stempro® Osteogenesis Differentiation kit medium is a specialized cell culture medium designed to support the differentiation of stem cells into osteoblasts, the cells responsible for bone formation. The medium contains a proprietary formulation of growth factors and supplements that promote the commitment and maturation of stem cells towards the osteogenic lineage.

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2 protocols using stempro osteogenesis differentiation kit medium

1

Osteogenic Differentiation of hMSCs

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hMSC were seeded at 5 × 103 cells/cm2 in α-MEM. After 24 h, cells were approximately 80% confluent and osteogenic differentiation was initiated by switching to commercially available Stempro® Osteogenesis Differentiation kit medium (Invitrogen) or osteogenic differentiation medium consisting of α-MEM, 15% FBS, 100 μM ascorbic acid (Scientific Lab Supplies, UK), 2.5 mM β-glycerophosphate (Calbiochem) and 100 nM dexamethasone (Sigma). For experiments on undifferentiated hMSC, cells were grown in α-MEM supplemented with 10% heat-inactivated foetal bovine serum (FBS, Sigma), 1000 U/mL penicillin, and 10 mg/mL streptomycin (Sigma). Medium was changed every 3–4 days, and cells were used until passage 8 only. VCP/p97 and the proteasome were inhibited with DBeQ (Biovision) or bortezomib (Calbiochem). Tunicamycin was purchased from Sigma.
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2

hMSC Osteogenic Differentiation Protocol

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hMSC were seeded at 5x103 cells/cm2 in α-MEM. After 24 h, cells were approximately 80% confluent and osteogenic differentiation was initiated by switching to commercially available Stempro® Osteogenesis Differentiation kit medium (Invitrogen) or osteogenic differentiation medium consisting of α-MEM, 15% FBS, 100 μM ascorbic acid (Scientific Lab Supplies, UK), 2.5 mM β-glycerophosphate (Calbiochem) and 100 nM dexamethasone (Sigma). For experiments on undifferentiated hMSC, cells were grown in α-MEM supplemented with 10% heat-inactivated foetal bovine serum (FBS, Sigma), 1000 U/mL penicillin, and 10mg/mL streptomycin (Sigma). Medium was changed every 3 to 4 days, and cells were used until passage 8 only. VCP/p97 and the proteasome were inhibited with DBeQ (Biovision) or bortezomib (Calbiochem). Tunicamycin was purchased from Sigma.
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