Pf 382

Manufactured by American Type Culture Collection

Sourced in United States

The PF-382 is a laboratory equipment product offered by American Type Culture Collection. It is designed for culturing cells and maintaining cell lines. The core function of the PF-382 is to provide a controlled environment for cell growth and proliferation.

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Lab products found in correlation

2 protocols using pf 382

Cell Line Cultivation Protocol

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PF-382, TALL-1, HPB-ALL, and Jurkat were purchased from DSMZ. CCRF-CEM and HEK293T cell lines were obtained from ATCC.
PF-382, TALL-1, CCRF-CEM, and Jurkat, were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% fetal bovine serum (Lonza Group Ltd., Basel, Switzerland), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific), and maintained at 37 °C in a 5% CO₂ atmosphere.
The HPB-ALL cell line was cultured in RPMI 1640 supplemented with 20% fetal bovine serum.
The HEK293T cell line was cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Riillo C., Caracciolo D., Grillone K., Polerà N., Tuccillo F.M., Bonelli P., Juli G., Ascrizzi S., Scionti F., Arbitrio M., Lopreiato M., Siciliano M.A., Sestito S., Talarico G., Galea E., Galati M.C., Pensabene L., Loprete G., Rossi M., Ballerini A., Gentile M., Britti D., Di Martino M.T., Tagliaferri P, & Tassone P. (2022). A Novel Bispecific T-Cell Engager (CD1a x CD3ε) BTCE Is Effective against Cortical-Derived T Cell Acute Lymphoblastic Leukemia (T-ALL) Cells. Cancers, 14(12), 2886.

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Culturing Leukemia Cell Lines

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Ke-37, PF-382, TALL-1, HPB-ALL, DND-41, MOLT-4, JURKAT, p12-ichikawa and ALL-SIL were purchased by DSMZ. CCRF-CEM cell lines was obtained by ATCC.
Ke-37, PF-382, TALL-1, DND-41, ALL-SIL, CCRF-CEM, MOLT-4, JURKAT, p12-ichikawa were cultured in RPMI-1640 (Gibco, Life Technologies, Carlsbad, California, USA), supplemented with 10% fetal bovine serum (Lonza Group, Basel, Switzerland), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco, Life Technologies), and maintained at 37°C in a 5% CO₂ atmosphere.
HPB-ALL cell line was cultured in RPMI-1640 supplemented with 20% fetal bovine serum.

Caracciolo D., Riillo C., Ballerini A., Gaipa G., Lhermitte L., Rossi M., Botta C., Duroyon E., Grillone K., Gallo Cantafio M.E., Buracchi C., Alampi G., Gulino A., Belmonte B., Conforti F., Golino G., Juli G., Altomare E., Polerà N., Scionti F., Arbitrio M., Iannone M., Martino M., Correale P., Talarico G., Ghelli Luserna di Rorà A., Ferrari A., Concolino D., Sestito S., Pensabene L., Giordano A., Hildinger M., Di Martino M.T., Martinelli G., Tripodo C., Asnafi V., Biondi A., Tagliaferri P, & Tassone P. (2021). Therapeutic afucosylated monoclonal antibody and bispecific T-cell engagers for T-cell acute lymphoblastic leukemia. Journal for Immunotherapy of Cancer, 9(2), e002026.

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