Human ip10 elisa set

Manufactured by BD

The BD Human IP10 ELISA set is a laboratory equipment used for the detection and quantitative measurement of human IP-10 (Interferon-gamma-induced Protein 10) in biological samples. It is designed to facilitate enzyme-linked immunosorbent assay (ELISA) experiments.

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6 protocols using human ip10 elisa set

Cytokine Profiling in MDM Cultures

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IP-10 in MDM culture supernatants was measured with a BD Human IP-10 ELISA Set (BD). Multiplex cytokine assay using a human cytokine 29-plex kit (Millipore) was performed using culture supernatants and was analyzed with a DropArray (Curiox Biosystems) and the MAGPIX System (Luminex). Bioactive IFN-I was measured by a bioassay using 293 ISRE-luc cell line (a gift from Drs. Junzhi Wang, National Institute for the Control of Pharmaceutical and Biological Products, China and Xuguang Li, University of Ottawa, Canada)⁴⁹.

Akiyama H., Miller C.M., Ettinger C.R., Belkina A.C., Snyder-Cappione J.E, & Gummuluru S. (2018). HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. Nature Communications, 9, 3450.

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ELISA-based Quantification of Immune Factors and Viral Production

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IP-10 and CCL2 production in culture supernatants was measured with a BD human IP-10 ELISA set and a BD human MCP-1/CCL2 ELISA set, respectively. To quantitate virus production, p24^Gag in culture supernatants was quantified by in-house ELISA (8 (link)).

Akiyama H., Jalloh S., Park S., Lei M., Mostoslavsky G, & Gummuluru S. (2021). Expression of HIV-1 Intron-Containing RNA in Microglia Induces Inflammatory Responses. Journal of Virology, 95(5), e01386-20.

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Quantifying CXCL10 in DM2 cell models

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RNA from DM2 patients was transfected into MDA5^-/- Hela cells. After 24 h the supernatant was harvested, and the concentration of CXCL10 was determined by ELISA (ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) #439904 BioLegend, San Diego, CA). For THP-1 experiments, the human IP10 ELISA set (BD Bioscience) was used.

Rösing S., Ullrich F., Meisterfeld S., Schmidt F., Mlitzko L., Croon M., Nattrass R.G., Eberl N., Mahlberg J., Schlee M., Wieland A., Simon P., Hilbig D., Reuner U., Rapp A., Bremser J., Mirtschink P., Drukewitz S., Zillinger T., Beissert S., Paeschke K., Hartmann G., Trifunovic A., Bartok E, & Günther C. (2024). Chronic endoplasmic reticulum stress in myotonic dystrophy type 2 promotes autoimmunity via mitochondrial DNA release. Nature Communications, 15, 1534.

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Quantification of IFNβ and CXCL10 Secretion

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IFNβ secreted to the supernatants of fibroblasts was quantified using the HEK-Blue^TM IFN-α/β reporter system by InvivoGen and normalized to the cell number. Cell number was determined by Hoechst 33258 staining. CXCL10 release was measured using the human IP10 ELISA set (BD Bioscience), performed according to the manufacturer’s instructions.

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Quantifying Human Cytokine Levels by ELISA

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For detection of human IL‐6 and IP‐10, commercially available ELISA kits were used (Human IL‐6 ELISA Set, BD OptEIA, 555220; Human IP‐10 ELISA Set, BD OptEIA, 550926) according to the manufacturer's instructions. Basal medium, used for NHBE culturing at time of treatment and infection, was used as blank control. Statistics (Fig 4E) were calculated using paired Student's two‐sided t‐test on log‐transformed values between indicated conditions before donor‐wise normalization to vehicle‐treated mock controls.

Bergant V., Yamada S., Grass V., Tsukamoto Y., Lavacca T., Krey K., Mühlhofer M., Wittmann S., Ensser A., Herrmann A., vom Hemdt A., Tomita Y., Matsuyama S., Hirokawa T., Huang Y., Piras A., Jakwerth C.A., Oelsner M., Thieme S., Graf A., Krebs S., Blum H., Kümmerer B.M., Stukalov A., Schmidt‐Weber C.B., Igarashi M., Gramberg T., Pichlmair A, & Kato H. (2022). Attenuation of SARS‐CoV‐2 replication and associated inflammation by concomitant targeting of viral and host cap 2'‐O‐ribose methyltransferases. The EMBO Journal, 41(17), e111608.

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Cytokine Measurement by ELISA

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Cytokine levels were measured by ELISA according to manufacturer's recommendations. The following kits were used: CXCL10 (IP-10): Human IP-10 ELISA Set; TNF: Human TNF ELISA Set; IL-6: Human IL-6 ELISA Set; IL-1β: Human IL-1β ELISA Set (all BD Biosciences); and IFN-γ: DuoSet Human IFN-γ (R&D Systems).

Boehmer D.F., Koehler L.M., Magg T., Metzger P., Rohlfs M., Ahlfeld J., Rack-Hoch A., Reiter K., Albert M.H., Endres S., Rothenfusser S., Klein C., Koenig L.M, & Hauck F. (2020). A Novel Complete Autosomal-Recessive STAT1 LOF Variant Causes Immunodeficiency with Hemophagocytic Lymphohistiocytosis–Like Hyperinflammation. The Journal of Allergy and Clinical Immunology. in Practice, 8(9), 3102-3111.

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