Anti cd95

Manufactured by Miltenyi Biotec

Sourced in Germany

Anti-CD95 is a lab equipment product from Miltenyi Biotec. It is a monoclonal antibody that binds to the CD95 (Fas) receptor on the surface of cells.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti cd95

Quantification of B Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

One week after IP challenge, splenocytes were isolated and single cell suspensions were stained for B cell subpopulations. Surface characterization was carried with monoclonal antibodies from Biolegend (anti-CD19, CD138, CD267, CD38, GL7, IgM, IgD, and IgG) and anti-CD95 from Miltenyi (Bergisch Gladbach, Germany) as described in Table S1. B cells were phenotyped using an Attune NxT flow cytometer (Thermo Fisher Scientific) (Figure S5) and analyzed with FCS express software (De Novo Software).

Gonzalez-Visiedo M., Li X., Munoz-Melero M., Kulis M.D., Daniell H, & Markusic D.M. (2022). Single-dose AAV vector gene immunotherapy to treat food allergy. Molecular Therapy. Methods & Clinical Development, 26, 309-322.

+ Open protocol

+ Expand

Comprehensive T-cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular composition, CD4/CD8 ratio and CAR-expression was determined by flow cytometric analysis after staining with one or several of the following antibodies and reagents: anti-CD45, anti-CD4, anti-CD3, anti-CD16, anti-CD56, anti-CD19, anti-CD14, anti-CD8, anti-CD45RO, anti-CD95, anti-CD62L and 7-AAD (all Miltenyi Biotec). CAR-modified T cells were identified using the EGFRt marker and staining with an anti-EGFRt antibody (clone: C225, ImClone Systems) conjugated in-house to AlexaFluor 647. Data were acquired on a MACSQuant Analyzer 10 and analyzed using MACSQuantify software (both Miltenyi Biotec).

Lock D., Monjezi R., Brandes C., Bates S., Lennartz S., Teppert K., Gehrke L., Karasakalidou-Seidt R., Lukic T., Schmeer M., Schleef M., Werchau N., Eyrich M., Assenmacher M., Kaiser A., Prommersberger S., Schaser T, & Hudecek M. (2022). Automated, scaled, transposon-based production of CAR T cells. Journal for Immunotherapy of Cancer, 10(9), e005189.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!