Six-week-old female C57BL/6 mice were purchased from Orient Bio (Korea). All mice were maintained in the animal care facility of KAIST according to the KAIST animal care and use guidelines (KA2013-09). Murine granulocyte-macrophage colony-stimulating factor (mGM-CSF) was acquired from R&D Systems (USA). Lipopolysaccharide (LPS; O111:B4) was obtained from Sigma-Aldrich (USA). FITC-conjugated anti-mouse MHCII, PE-labeled anti-mouse CD80, and CD86 antibodies were purchased from eBioscience (USA). Polyclonal anti-Mfn1 and anti-Mfn2 antibodies were kindly provided by Dr. Richard J. Youle. Antibodies for cytochrome c, OPA1, Drp1, Tom20, APC-labeled CD11c, and CD16/CD32 were obtained from BD Biosciences (USA).
Lipopolysaccharide lps o111 b4
Manufactured by Merck Group
Sourced in United States, Macao
Lipopolysaccharide (LPS; O111:B4) is a laboratory reagent derived from the cell walls of Escherichia coli. It functions as a potent activator of the immune system, triggering inflammatory responses in mammalian cells.
Automatically generated - may contain errors
Lab products found in correlation
Sytox green, by Thermo Fisher Scientific (3 mentions)
Cd16 cd32, by BD (1 mentions)
Mgm csf, by R&D Systems (1 mentions)
Pe labeled anti mouse cd80, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti mouse mhcii, by Thermo Fisher Scientific (1 mentions)
C57bl 6 mice, by Orient Bio (1 mentions)
Cp 456773, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
Anti tnf α mab11, by BD (1 mentions)
Anti il 10 jes3 9d7, by BioLegend (1 mentions)
Anti cd14 mφ p9, by BD (1 mentions)
Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions)
Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
2 protocols using lipopolysaccharide lps o111 b4
1
Mitochondrial Dynamics in Murine Dendritic Cells
2
Inflammasome Activation and Cytokine Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Uric acid, lipopolysaccharide (LPS) (O111:B4), and NLRP3 inflammasome inhibitor (CP-456773) were purchased from Sigma (St Louis, MO). Quant-iT PicoGreen dsDNA Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA). RPMI-1640, penicillin-streptomycin, and fetal bovine serum were obtained from WelGene (Daejeon, South Korea). For multi-color flow cytometry, the following antibodies were used: Fixable Viability Dye eFluor® 506, anti-CD3 (UCHT-1), anti-CD19 (HIB19), anti-CD56 (B159), and anti-IL-1RA (CRM17) (All from eBioscience, San Diego, CA, USA); anti-CD14 (MφP9), anti-IL-1β (AS10), and anti-TNF-α (Mab11) (All from BD Bioscience, San Jose, CA); anti-IL-10 (JES3-9D7) and anti-IL-8 (6217) (Both from Biolegend, San Diego, CA); anti-TGF–β1 (27232) (R&D Systems, Minneapolis, MN). For immunofluorescence, SYTOX Green and anti-MPO (4A4) were obtained from Thermo Fisher Scientific (Waltham, MA). Anti-NE (NP57) and anti-citrullinated histone H3 (rabbit polyclonal) were obtained from Santa Cruz Biotechnology Inc. (Dallas, Texas) and Abcam (Cambridge, MA), respectively.
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