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Annexin 5 alexa fluor 488 and 647 conjugates

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Annexin V-Alexa Fluor-488 and -647 conjugates are fluorescent-labeled proteins that bind to phosphatidylserine, a phospholipid exposed on the surface of apoptotic cells. These conjugates can be used to detect and quantify apoptosis in cell populations.

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Lab products found in correlation

Rabbit anti caspase 7, by Cell Signaling Technology (2 mentions) Caspase 7, by Merck Group (2 mentions) Rabbit anti annexin a2, by BD (2 mentions) Rabbit anti listeria, by BD (2 mentions) Rabbit anti lm antibody, by Abcam (2 mentions) Mouse anti phosphatidylserine, by Abcam (2 mentions) Mouse anti ezrin, by Thermo Fisher Scientific (2 mentions) Fluorescent secondary antibodies alexafluor conjugates, by Thermo Fisher Scientific (2 mentions) Annexin a1, by Merck Group (2 mentions) Annexin a2, by Merck Group (2 mentions) Annexin a6, by Merck Group (2 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) X tremegene 9, by Roche (2 mentions)

2 protocols using annexin 5 alexa fluor 488 and 647 conjugates

1

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

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Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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2

Fluorescence Microscopy Protocols for Annexin and Listeria Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were rabbit anti-Annexin A2 (#610068 from BD Biosciences), rabbit anti-GFP (#A11120 from Invitrogen), rabbit anti-Caspase 7 (#12827 from Cell Signaling), rabbit anti-Listeria (#B223021 from BD Biosciences), rabbit anti-Lm antibody (from Dr. Pascale Cossart, Institut Pasteur, France), mouse anti-phosphatidylserine (#18005 from Abcam) and mouse anti-ezrin (#35-7300 from Invitrogen). Rat anti-TIM4 blocking antibody was previously described37 . Alexa Fluor 568 Phalloidin, Annexin V-Alexa Fluor-488 and -647 conjugates and all fluorescent secondary antibodies (AlexaFluor conjugates) were from Invitrogen. DAPI (#D1306 from Invitrogen) was used at 1:2500 dilution to stain the nuclei where indicated. Cytochalasin D (#2502555; 10 μM final) and Latrunculin B (#428020; 10μg/mL final) were from Calbiochem. For transfection of HeLa cells, Xtreme Gene 9 (Roche) transfection reagent was used as per manufacturer’s protocols. LifeAct-mRFP38 was a gift from Dr. Ray Truant (McMaster University, Canada). The following siRNAs were from Sigma: Annexin A1 (#00157996), Annexin A2 (#00246294), Annexin A6 (#00063383), Caspase 7 (#00128361).
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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