Mouse monoclonal antibody to actin

Manufactured by Merck Group

Mouse monoclonal antibody to actin is a laboratory reagent used to detect and quantify the presence of actin, a structural protein found in all eukaryotic cells. This antibody can be used in various immunoassay techniques to identify and measure actin levels in biological samples.

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Lab products found in correlation

Nanog, by Cell Signaling Technology (1 mentions) Aicar, by Beyotime (1 mentions) Rapamycin, by Sangon (1 mentions) Mg132, by Beyotime (1 mentions)

2 protocols using mouse monoclonal antibody to actin

Pluripotency Marker Expression Analysis

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The antibodies used are as follows: Sox2 (#3579), Oct4 (# 2750), Nanog (#4903), acetylated histone H3 Lysine 9 (# 9671), and Gli1 (#2643) were from Cell Signaling Technologies; mouse monoclonal antibody to actin was from Sigma-Aldrich; Gli2 (SC-271786) was purchased from Santa Cruz Biotechnologies; and Gli3 antibody (Ab55437) was from Abcam.

Bora-Singhal N., Perumal D., Nguyen J, & Chellappan S. (2015). Gli1-Mediated Regulation of Sox2 Facilitates Self-Renewal of Stem-Like Cells and Confers Resistance to EGFR Inhibitors in Non–Small Cell Lung Cancer. Neoplasia (New York, N.Y.), 17(7), 538-551.

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Regulation of Autophagy and Signaling

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If not otherwise indicated, all chemicals were from Sigma. MG132 (10 μM; Beyotime Biotechnology) and CQ (100 μM) exposure was for 4 hr. Rapamycin (200 nM; Sangon Biotech), AICAR (500 μM; Beyotime Biotechnology), MHY (5 μM) and dorsomorphin (5 μM) were added to the medium for 12 hr. pAC5.1-flag and pAC5.1-HA vectors were kindly provided by Xiaohang Yang (Zhejiang University). AKHR-HA was made by cloning the cDNA of AKHR into a pAC5.1-HA vector using SalI and NotI. The cDNA of GFP-ATG8 was from Chao Tong (Zhejiang University) and cloned into a pAC5.1-HA vector using EcoRI and BamHI to generate HA-GFP-ATG8.
The following antibodies were used: rabbit polyclonal antibodies to p-AKT, p-AMPK, HA, Flag, GFP and mouse monoclonal antibody to HA (Cell Signaling Technology); mouse monoclonal antibody to actin (sigma); rabbit polyclonal antibody to AKH (biorbyt); rabbit polyclonal antibody to Dilp2 was kindly provided by Zhefeng Gong (Zhejiang University).

Huang R., Song T., Su H., Lai Z., Qin W., Tian Y., Dong X, & Wang L. (2020). High-fat diet enhances starvation-induced hyperactivity via sensitizing hunger-sensing neurons in Drosophila. eLife, 9, e53103.

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