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3 protocols using reagent grade chloroform

1

Synthesis and Characterization of Metal Nitrate Compounds

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The AgNO3 (99.8%), Co(NO3)2·6H2O (99.0%), Cu(NO3)2·3H2O (99.5%), Ni(NO3)2·6H2O (99.0%), Pb(NO3)2 (99.5%) nitrate salts were purchased from Merck, Zn(NO3)2·6H2O (98.0%) and Cd(NO3)2·4H2O (98.0%) were purchased from Aldrich. For preparation of PTC, phosphorus pentachloride (98%), trifluoroacetamide (97%), benzylamine (99%) and formic acid (97%) were used. Reagent grade chloroform (99.4%), dichloromethane (99.8%), 1,2-dichloroethane (99.8%) and nitrobenzene (99%) were tested as organic phases from Merck company; all chemicals were of the highest purity available and used without further purification. Doubly distilled deionized water was used throughout all experiments.
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2

UHPLC-MS Analysis of Flavonoids

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UHPLC-MS Solvents, LC-MS formic acid and reagent grade chloroform were from Merck (Santiago, Chile). Ultrapure water was obtained from a Millipore water purification system (Milli-Q Merck Millipore, Chile). HPLC standards, (kaempferol, quercetin, isorhamnetin, eriodictyol, luteolin, apigenin, naringenin, all standards with purity higher than 95 % by HPLC) were purchased either from Sigma Aldrich (Saint Louis, Mo, USA), ChromaDex (Santa Ana, CA, USA), or Extrasynthèse (Genay, France). Folin-Ciocalteu phenol reagent (2N), reagent grade Na2CO3, AlCl3, HCl, FeCl3, NaNO2, NaOH, quercetin, trichloroacetic acid, sodium acetate, Gallic acid, Trolox, ABTS and potassium persulfate, xanthine oxidase and DPPH (1,1-diphenyl-2-picrylhydrazyl radical) were purchased from Sigma-Aldrich Chemical Co. (Santiago, Chile).
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3

UHPLC-MS Analytical Protocols

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UHPLC-MS Solvents, LC-MS formic acid, reagent grade lanzoprasole, formalin, ethanol, HCl, deuterated chloroform and deuterated methanol, and reagent grade chloroform were from Merck (Santiago, Chile). Ultrapure water was obtained from a Millipore water purification system (Milli-Q Merck Millipore, Santiago, Chile). UHPLC standards, (kaempferol, caffeic acid, isorhamnetin, hesperetin, eriodictyol, all standards with purity higher than 95% by HPLC) were purchased either from Sigma Aldrich (Saint Louis, Mo, USA), ChromaDex (Santa Ana, CA, USA), or Extrasynthèse (Genay, France).
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