Sodium azide

Manufactured by Labsynth

Sourced in Brazil

Sodium azide is a colorless, crystalline compound that is commonly used as a laboratory reagent. It has the chemical formula NaN3. Sodium azide is a stable, non-flammable substance that is soluble in water.

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Lab products found in correlation

2 protocols using sodium azide

Glutathione Reductase Activity Assay

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The methodology was based on the technique described by Paglia and Valentine [41 ]. Briefly, 10 μL of sample and 130 μL of solution 1 (0.1 M sodium phosphate buffer, pH 7.0, 3.07 mM sodium azide (Labsynth^®, Diadema, SP, Brazil), 0.30 mM NADPH (Sigma-Aldrich^®, St. Louis, MO, USA), 3.07 mM GSH, and 1.54 U/mL glutathione reductase (Sigma-Aldrich^®, St. Louis, MO, USA) were added to microtubes and incubated for 2 min at room temperature. Afterwards, 60 μL of solution 2 was added (5 mM hydrogen peroxide (Neon^®, Suzano, SP, Brazil) in 0.1 M sodium phosphate buffer, pH 7.0). The absorbance reading was performed at 340 nm for 5 min, with one-minute intervals between each reading. Enzyme activity was expressed in nmol/min/mg of protein.

Galiciolli M.E., Silva J.F., Prodocimo M.M., Laureano H.A., Calado S.L., Oliveira C.S, & Guiloski I.C. (2023). Toxicological Effects of Thimerosal and Aluminum in the Liver, Kidney, and Brain of Zebrafish (Danio rerio). Metabolites, 13(9), 975.

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Ecobidens Glycolic Extract Anticancer Evaluation

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Ecobidens^® (B. pilosa glycolic extract, BPE) and butylated hydroxytoluene were obtained from Chemyunion (Sorocaba, SP, Brazil) and Mapric (São Paulo, SP, Brazil), respectively. Polyethylene glycol l400, propylene glycol and sodium azide were acquired from Labsynth (Diadema, SP, Brazil). 5-Fluorouracil, hexadecyltrimethylammonium bromide, poloxamer 407, bovine serum albumin (BSA), n-butanol and ortho-dianisidine were purchased from Sigma–Aldrich (St. Louis, MO, USA). ImmunoCruz™ mouse ABC staining systems (sc-2017 and 2018), monoclonal mouse anti-mouse p53 (clone 3H2820) and polyclonal rabbit anti-mouse Bax (clone P-19) antibodies were acquired from Santa Cruz Biotechnology (CA, USA), whereas 3,3 diaminobenzidine (DAB), Duet strept ABC complex/HRP kit 0492 and anti-human Bcl-2 (clone 124) antibodies were obtained from Dako (Carpinteria, CA, USA). Anti-human ki-67 (clone MM1) antibodies were acquired from Novocastra (Newcastle, UK). Trichloroacetic acid, hydrogen peroxide (H₂O₂), tris, hydrochloricacid (HCl), methanol and xylene were obtained from Vetec (Rio de Janeiro, RJ, Brazil). Thiobarbituric acid, hematoxylin and eosin staining were acquired from Merck (Darmstadt, HE, Germany). Xylazine and ketamine were purchased from Syntec (Cotia, SP, Brazil) and König (Santana de Parnaíba, SP, Brazil), respectively.

de Ávila P.H., de Ávila R.I., dos Santos Filho E.X., Cunha Bastos C.C., Batista A.C., Mendonça E.F., Serpa R.C., Marreto R.N., da Cruz A.F., Lima E.M, & Valadares M.C. (2015). Mucoadhesive formulation of Bidens pilosa L. (Asteraceae) reduces intestinal injury from 5-fluorouracil-induced mucositis in mice. Toxicology Reports, 2, 563-573.

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