Q card 2 system

Ethanol was obtained from Merck. Dimethylamine, α-cyano-4-hydroxycinnamic acid, dimethylsulfoxide, 1-hydroxybenzotriazole hydrate, sodium hydroxide, and TFA were obtained from Sigma-Aldrich. HPLC SupraGradient acetonitrile (ACN) was obtained from Biosolve. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide from Fluorochem. Recombinant peptide N-acetyl-beta-glucosaminyl asparagine amidase PRIME-LY Glycosidase from Flavobacterium meningosepticum was purchased from N-Zyme Scientifics. The peptide calibration standard was purchased from Bruker Daltonics. All buffers were prepared using Milli-Q water (mQ) generated from a Q-card 2 system (Millipore).

Sodium bicarbonate, glacial acetic acid (HAc), hydrogen peroxide and ethanol (EtOH) were obtained from Merck (Darmstadt, Germany). α-cyano-4-hydroxycinnamic acid (CHCA), 2-picoline borane, dimethylsulfoxide (DMSO), 1-hydroxybenzotriazole hydrate (HOBt), 50% sodium hydroxide, ammonium acetate, and trifluoroacetic acid (TFA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC SupraGradient acetonitrile (ACN) was obtained from Biosolve (Valkenswaard, The Netherlands). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and hydrochloride acid was obtained from Fluorochem (Hadfield, UK). Recombinant PNGase F PRIME-LY™ Glycosidase from Flavobacterium meningosepticum was purchase from N-Zyme Scientifics (Doylestown, PA, USA). Peptide calibration standard II was purchased from Bruker Daltonics (Bremen, Germany). All buffers were prepared using deionized water (mQ) generated from a Q-card 2 system (Millipore, The Netherlands). Poly-horseradish peroxidase solution (Poly-HRP) was obtained from Immunologic (Duiven, The Netherlands). 3,3’-diaminobenzidine (DAB) was obtained from DAKO (Agilent Technologies, Santa Clara, CA, USA).