LIVE/DEAD Fixable Blue Viability Dye (Invitrogen), CD25 BV421 (clone M-A251, BD), CD3 BUV661 (clone UCHT1, BD Biosciences), CD8 BUV805 (clone SK1, BD Biosciences), CD4 APCR700 (clone RPA-T4, BD Biosciences), CD161 BV605 (clone DX12, BD), Vα7.2 PE (clone 3C10, BioLegend), IL-7Rα BV786 (clone HIL-7R-M21, BD Biosciences), Foxp3 Alexa Fluor 488 (clone 259D/C7, BD Biosciences), Helios PerCP-eFluor 710 (clone 22F6, Invitrogen), CTLA-4 PECF594 (BNI3, BD Biosciences).
Cd25 bv421 clone m a251
Manufactured by BD
CD25 BV421 is a fluorochrome-conjugated monoclonal antibody that binds to the CD25 antigen. CD25 is the alpha chain of the interleukin-2 receptor, which is expressed on activated T cells and regulatory T cells. The BV421 fluorochrome is a violet-excited, blue-emitting dye that can be used in multicolor flow cytometry experiments.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable blue viability dye, by Thermo Fisher Scientific (1 mentions)
Cd8 buv805 clone sk1, by BD (1 mentions)
Vα 7.2 pe clone 3c10, by BioLegend (1 mentions)
Foxp3 alexa fluor 488 clone 259d c7, by BD (1 mentions)
Ctla 4 pe cf594 bni3, by BD (1 mentions)
Cd4 apc r700 clone rpa t4, by BD (1 mentions)
Fixable viability stain 510, by Thermo Fisher Scientific (1 mentions)
Cd45ro bv421 clone uchl1, by BD (1 mentions)
P24 kc57 fitc, by Beckman Coulter (1 mentions)
Cd4 pe cy7 clone l200, by BD (1 mentions)
Cd69 apc, by BioLegend (1 mentions)
Cd4 bv786 clone sk3, by BD (1 mentions)
Cd27 bv605 clone l128, by BD (1 mentions)
Cd3 af700 clone ucht1, by BD (1 mentions)
Cd69 percp cy5 5 clone fn50, by BD (1 mentions)
2 protocols using cd25 bv421 clone m a251
1
Comprehensive Immunophenotyping of T-cell Subsets
2
Multiparameter Flow Cytometry Panel
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Fixable Viability Stain 510 was obtained from ThermoFisher Scientific (#L34957, 1/1000). The following antibodies were used in staining experiments: CD3 AF700 Clone UCHT1 (BD Biosciences, #557943, 1/50), CD4 PE-Cy7 Clone L200 (BD Bioscience, #560644, 1/200) and CD4 BV786 Clone SK3 (BD Biosciences, #563881, 1/200), CD8 BV510 Clone RPA-T8 (BioLegend, #301047, 1/200), CD45RO BV421 Clone UCHL1 (BD Biosciences, #562649, 1/100) or CD45RO PE Clone HI100 (BD Biosciences, #5555493, 1/20), CD27 BV605 Clone L128 (BD Biosciences, #562656, 1/100), PD1 BB700 Clone EH12.1 (BD Biosciences, #566461, 1/100), CD69 PerCP-Cy5.5 Clone FN50 (BD Biosciences, #560738) and CD69 APC (BioLegend, #310909, 1/50), CD25 BV421 Clone M-A251 (BD Biosciences, #562443, 1/50), HLA-DR FITC Clone REA805 (Miltenyi Biotech, #130-111-788, 1/50), GZMA PE-Cy7 Clone CB9 (BioLegend, #507221, 1/25), GZMB PB Clone GB11 (BioLegend, #515407, 1/50), CCL5 PerCP-Cy5.5 Clone VL1 (BioLegend, #515507, 1/50), CD127 PE-CF594 Clone HIL-7R-M21 (BD Biosciences, #562397, 1/50). For p24 staining, we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, #6604665, 1/500) and p24 28B7-APC (MediMabs, #MM-0289-APC, 1/400).
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