Total flavonoid content was estimated using the method reported by Chandra et al. [22 ]. CCE was diluted to 1 mg/mL with 80% ethanol, and 100 μL of this solution was mixed with 100 μL of 2% aluminum (III) chloride hexahydrate (Sigma-Aldrich Co., Saint Louis, MO, USA). Quercetin (Sigma-Aldrich Co., Saint Louis, MO, USA) was diluted in 80% ethanol from 2.5 μg/mL to 100 μg/mL. After mixing the diluted standard solution with 100 μL of 2% aluminum (III) chloride hexahydrate, the mixture was incubated at room temperature for 10 min and then analyzed at 430 nm wavelength using a microplate reader.
Aluminum 3 chloride hexahydrate
Manufactured by Merck Group
Sourced in United States
Aluminum(III) chloride hexahydrate is a chemical compound with the formula AlCl3·6H2O. It is a white, crystalline solid that is commonly used in various laboratory applications. The core function of this product is to serve as a Lewis acid, a type of electron-pair acceptor, which can be utilized in a range of chemical reactions and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Iron 3 chloride hexahydrate, by Merck Group (2 mentions)
Quercetin, by Merck Group (1 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Manganese 2 chloride tetrahydrate, by Merck Group (1 mentions)
Iron 2 chloride tetrahydrate, by Merck Group (1 mentions)
Magnesium 2 chloride hexahydrate, by Merck Group (1 mentions)
Isatoic anhydride, by Merck Group (1 mentions)
Chloroacetyl chloride, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate, by Merck Group (1 mentions)
Dibenzoylmethane, by Merck Group (1 mentions)
Nickel 2 chloride hexahydrate, by Merck Group (1 mentions)
Mercury 2 chloride, by Merck Group (1 mentions)
Cadmium 2 chloride, by Merck Group (1 mentions)
Copper 2 chloride dihydrate, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Lead 2 chloride, by Merck Group (1 mentions)
Zinc 2 chloride, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
3 protocols using aluminum 3 chloride hexahydrate
1
Total Flavonoid Content Estimation
2
Trace Metal Analysis Workflow
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4-Phenylazo-m-phenylenediamine (Chrysoidine G, CG), lithium chloride, iron(II) chloride tetrahydrate, iron(III) chloride hexahydrate, magnesium(II) chloride hexahydrate, manganese(II) chloride tetrahydrate, and aluminum(III) chloride hexahydrate was purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Cobalt standard solutions were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Standard copper, zinc, arsenic, cadmium, and mercury solutions were purchased from CPI International, Co. (Santa Rosa, CA, USA). All reagents and chemicals were of analytical grade and were prepared using highly pure water with a resistivity of 18 MΩ·cm.
3
Heavy Metal Analysis in Environmental Samples
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The aluminum cans were obtained from commercial shops (Mansoura, Egypt). Isatoic anhydride, dibenzoylmethane, 4-amino-2-hydroxy benzoic acid, toluene, cadmium(ii ) chloride, chloroacetyl chloride, nickel(ii ) chloride hexahydrate, ethylenediaminetetraacetic acid tetrasodium salt dihydrate, zinc(ii ) chloride, aluminum(iii ) chloride hexahydrate, iron(iii ) chloride hexahydrate, and lead(ii ) chloride were obtained from Sigma-Aldrich. Hydrochloric acid, potassium chloride, sodium hydroxide, mercury(ii ) chloride, and copper(ii ) chloride dihydrate were purchased from Merck (Germany). The other chemicals were of analytical grade, obtained from Fluka (Switzerland) and were used without further purification unless otherwise stated. In addition, all aqueous solutions in this work were prepared by using doubly distilled water (resistance: 18.2 MΩ at 25 °C). It is worth mentioning that all glassware was rinsed in concentrated nitric acid solution (1% (v/v)) and washed with doubly distilled water before use. For analytical applications, the cucumber, lettuce leaves, and wastewater samples were collected from different agricultural locations in Samanoud (El-Gharbia).
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