Atcc htb 161

OVCAR3 cells expressing sfRon (OVCAR3-sfRon) were generated in similar way as MCF7-sfRon cells described elsewhere (4). OVCAR3 cell lines were cultured and maintained in RPMI 1640 medium (Gibco) with 10% fetal bovine serum (Gibco) at 37°C in a 5% CO₂ incubator. Our parental OVCAR3 (NIH-OVCAR3) cell line was purchased from ATCC (NIH: OVCAR-3, ATCC® HTB-161™) on 4/10/2012. ATCC authenticates their cell lines by short tandem repeat (STR) profiling analysis. To ensure the identity and validity of our cell lines and to prevent potential problems associated with cell culture, such as cell line misidentification, contamination and genetic drift, we purchase cell lines form validated, reliable source (e.g. ATCC) and cryopreserve 20 1 ml vials of each cell line at low passage (passage 1-3). The vials of low passage cell lines are kept protected in lab cell line bank and distributed to lab members according to the experimental needs. Recently, we performed authentication of several cell lines used in our recent published work (Bieniasz et. al., 2015) such as MCF7 and MCF7-sfRon, which have been in use in our lab for 8 years. Authentication performed by ATCC revealed that all tested cell lines showed 100% match to the original cell lines from which they were derived.