Quorum wavefx x1

Manufactured by Quorum Technologies

Sourced in Canada

The Quorum WaveFX-X1 is a specialized laboratory equipment designed for precise and controlled waveform generation. It provides users with the ability to create and manipulate various waveforms, such as sinusoidal, square, and triangular signals, with a high degree of accuracy and flexibility. The core function of the Quorum WaveFX-X1 is to generate these waveforms, which can be utilized in a wide range of scientific and engineering applications.

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Lab products found in correlation

Andor csu w1, by Oxford Instruments (3 mentions) Mosaic digital mirror forphotoactivation, by Oxford Instruments (2 mentions) Imageem camera, by Hamamatsu Photonics (2 mentions) Axioobserver z1 microscope, by Quorum Technologies (2 mentions) Alexa fluor 647 goat anti rabbit igg h l, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 goat anti mouse igg h l, by Thermo Fisher Scientific (2 mentions) Mouse 3d4 anti met, by Thermo Fisher Scientific (2 mentions) Aqua poly mount, by Polysciences (2 mentions) Dmem without phenol red, by Thermo Fisher Scientific (1 mentions) Glass bottomed 35 mm petri dishes, by Electron Microscopy Sciences (1 mentions) Metamorph, by Molecular Devices (1 mentions) Metamorph acquisition software, by Molecular Devices (1 mentions) Dmi6000b, by Leica (1 mentions) Pngase f, by New England Biolabs (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) μ dish, by Ibidi (1 mentions) Metamorph microscopy automation image analysis software, by Molecular Devices (1 mentions) Alexa fluor 568 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Phalloidin tritc, by Merck Group (1 mentions)

Close spelling variants of this product

WaveFX (21 citations) Quorum WaveFX (13 citations) WaveFX-X1 (5 citations)

12 protocols using quorum wavefx x1

Spinning-Disk Confocal Imaging with Photoactivation

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Images were acquired using Quorum WaveFX-X1 spinning-disk confocal
system (Quorum Technologies, Canada) equipped with Mosaic digital mirror for
photoactivation (Andor Technology, Connecticut) and Hamamatsu ImageEM camera
(Bridgewater, New Jersey) as previously described (14 (link)).

Bakhoum S.F., Kabeche L., Murnane J.P., Zaki B.I, & Compton D.A. (2014). DNA damage response during mitosis induces whole chromosome mis-segregation. Cancer discovery, 4(11), 1281-1289.

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Live Imaging of Embryonic Mandibular Arch

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Live image acquisition was performed by submerging embryos were submerged in 50% rat serum in DMEM without phenol red (Invitrogen) in a 25 mm imaging chamber. Cheese cloth was used to immobilise the embryo and position the mandibular arch directly against the coverglass. Embryos were imaged in a humidified chamber at 37 °C in 5% CO₂^{7 (link),23 (link)}. Time-lapse images were acquired on a Quorum WaveFX-X1 spinning disk confocal system (Quorum Technologies Inc.) at 20× magnification. Images were processed with Volocity software or ImageJ/Fiji. Representative images are shown from at least three independent experiments for each condition, and unless otherwise indicated, from at least three independent cohorts. No statistical method was used to predetermine sample size. Experiments were not randomised. Investigators were not blinded to allocation during experiments and outcome assessment.

Tao H., Zhu M., Lau K., Whitley O.K., Samani M., Xiao X., Chen X.X., Hahn N.A., Liu W., Valencia M., Wu M., Wang X., Fenelon K.D., Pasiliao C.C., Hu D., Wu J., Spring S., Ferguson J., Karuna E.P., Henkelman R.M., Dunn A., Huang H., Ho H.Y., Atit R., Goyal S., Sun Y, & Hopyan S. (2019). Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch. Nature Communications, 10, 1703.

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Imaging and Tracking Embryonic Development

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Embryos were anesthetized with 0.01% 3-aminobenzoic acid ester (Tricaine), immersed in 0.8% low-melting point agarose and mounted in glass-bottomed 35 mm Petri dishes (Electron Microscopy Sciences). After mounting, the Petri dish was filled with egg water containing PTU and Tricaine. A 40× water objective (NA = 1.1) mounted on a motorized Zeis AxioObserver Z1 microscope equipped with a Quorum WaveFX-X1 (Quorum Technologies) or Andor CSU-W1 (Oxford Instruments) spinning disc confocal system was used to capture all images. Images were imported into MetaMorph (Molecular Devices) and/or ImageJ. Time-lapse images were analyzed using cell tracking software as previously described in (Wang et al. 2018 (link)). All images were then imported in Adobe Photoshop and Illustrator. Adjustments were limited to levels, contrast, and cropping.

Ali M.F., Latimer A.J., Wang Y., Hogenmiller L., Fontenas L., Isabella A.J., Moens C.B., Yu G, & Kucenas S. (2021). Met is required for oligodendrocyte progenitor cell migration in Danio rerio. G3: Genes|Genomes|Genetics, 11(10), jkab265.

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Immunohistochemical Analysis of Embryonic Tissues

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Embryos and larvae were fixed in 4% PFA for 2 h at RT. After fixation, the embryos were sectioned by embedding them in 1.5% agarose/30% sucrose and frozen in 2-methylbutane chilled by immersion in liquid nitrogen. We collected 20 μm transverse sections on microscope slides using a cryostat microtome. Sections were rehydrated in PBS for 1 h and blocked with 5% goat serum/PBS for 1 h at RT. Primary antibody incubation was done overnight at 4°C. Secondary antibody incubation was done for 2 h at RT. Antibodies used were: rabbit anti-Sox10 (1:5000) (Binari et al. 2013 (link)), mouse 3D4 anti-Met (1:100; ThermoFisher), Alexa Fluor 647 goat anti-rabbit IgG(H+L) (1:1000), and Alexa Fluor 568 goat anti-mouse IgG(H+L) (1:1000). Sections were covered with Aqua-Poly/Mount (Polysciences). A 63× oil objective (NA = 1.4) mounted on a motorized Zeiss AxioObserver Z1 microscope equipped with a Quorum WaveFX-X1(Quorum Technologies) or Andor CSU-W1 (Andor Oxford Instruments) spinning disc confocal system was used to capture all images. Images were imported into Image J and Adobe Photoshop and Illustrator. Adjustments were limited to levels, contrast, and cropping.

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Multimodal Microscopy Imaging Protocol

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Confocal images were acquired using two different spinning-disk confocal microscope systems. The first was a Quorum Wave FX-X1 spinning-disk confocal microscope system (Quorum Technologies, Inc.) consisting of an inverted fluorescence microscope (DMI6000B; Leica Microsystems) equipped with a Hamamatsu ORCA-R² camera (C10600-10B) and a Hamamatsu ImagEM Enhanced EM charge-coupled device (CCD) camera (Hamamatsu Corporation). The second was a Quorum Diskovery spinning-disk confocal microscope system (Quorum Technologies, Inc.) consisting of an inverted fluorescence microscope (DMi8; Leica) equipped with the Andor Zyla 4.2-megapixel scientific complementary metal–oxide–semiconductor and Andor iXON 897 EM-CCD camera (Oxford Instruments). We also used an inverted microscope (IX81; Olympus Life Science) equipped with a Rolera-MGI Plus EM-CCD camera (ROL-MGi-PLUS-F-M-14-C; Q Imaging). The microscope systems were controlled by MetaMorph acquisition software (Molecular Devices). Images were acquired using a 63× oil immersion objective (1.4 NA), a 40× oil immersion objective (1.3 NA), or a 40× dry objective (0.60 NA). For fixed-cell imaging, Dako-mounted coverslips were imaged at RT. For live-cell imaging, coverslips were imaged in a chamber containing DMEM supplemented with 10% FBS in a microscope-mounted chamber maintained at 37°C and 5% CO₂.

Lancaster C.E., Fountain A., Dayam R.M., Somerville E., Sheth J., Jacobelli V., Somerville A., Terebiznik M.R, & Botelho R.J. (2021). Phagosome resolution regenerates lysosomes and maintains the degradative capacity in phagocytes. The Journal of Cell Biology, 220(9), e202005072.

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Confocal Microscopy Localization of Mutants

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Confocal microscopy was performed to verify the localization of mutants within cultured cells. In brief, HEK293 cells were seeded on poly-L-lysine-coated glass coverslips at 1 Â 10 6 cells/well and transfected 24 hours later with Lipofectamine 2000 and 4 mg of pcDNA3.1-containing mutants. After 6 hours of incubation at 37°C, media was replaced with Dulbecco's modified Eagle's medium containing 50 mM sodium butyrate and incubated at 28°C for a further 16 hours. Cells on coverslips were washed with phosphate-buffered saline (PBS), pH 7.4, then fixed with 95% cold (270°C) ethanol for 10 minutes. 3Cys DMRP1 proteins were detected using MRPr1 mAb as described above (Westlake et al., 2003) , coupled with Alexa 488 fluorescent anti-rat secondary antibody. Nuclei were stained with Hoechst 33342 (1:5000). Cells were examined under a Quorum WaveFX-X1 spinning disk confocal microscope (Quorum Technologies Inc., Guelph, ON, Canada).
To assess the degree of glycosylation, all MRP1 mutants were subjected to peptide:N-glycosidase F (PNGase F) treatment. Membrane vesicles containing 10 mg of total protein of 3Cys DMRP1 mutants were treated with 1 ml PNGase F (500 IU; New England Biolabs Ltd., Whitby, ON, Canada) according to manufacturer instructions. After treatment, protein samples were resolved by 7.5% SDS-PAGE and probed with an anti-His tag antibody.

Trofimova D.N, & Deeley R.G. (2018). Structural Studies of Multidrug Resistance Protein 1 Using "Almost" Cysless Template. Drug metabolism and disposition: the biological fate of chemicals, 46(6).

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Immunohistochemistry of Embryonic Sections

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Embryos and larvae were fixed in 4% PFA for 2 hr at RT. After fixation, the embryos were sectioned by embedding them in 1.5% agarose/30% sucrose and frozen in 2methylbutane chilled by immersion in liquid nitrogen. We collected 20 μm transverse sections on microscope slides using a cryostat microtome. Sections were rehydrated in PBS for 1 hr and blocked with 5% goat serum/PBS for 1 hr at RT. Primary antibody incubation was done overnight at 4C. Secondary antibody incubation was done for 2 hr at RT. Antibodies used were: rabbit anti-Sox10 (1:5000) (Binari et al. 2013) (link), mouse 3D4 anti-met (1:100; ThermoFisher), Alexa Fluor 647 goat anti-rabbit IgG(H+L)(1:1000), and Alexa Fluor 568 goat anti-mouse IgG(H+L) (1:1000). Sections were covered with Aqua-Poly/Mount (Polysciences). A 63X oil objective (NA = 1.4) mounted on a motorized Zeiss AxioObserver Z1 microscope equipped with a Quorum WaveFX-X1(Quorum Technologies) or Andor CSU-W1 (Andor Oxford Instruments) spinning disc confocal system was used to capture all images. Images were imported into Image J and Adobe Photoshop and Illustrator. Adjustments were limited to levels, contrast, and cropping.

Ali M.F., Latimer A.J., Wang Y., Hogenmiller L., Fontenas L., Isabella A.J., Moens C.B., Yu G., & Kucenas S. (2021). Met is required for oligodendrocyte progenitor cell migration in Danio rerio.

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HER2-Mediated EGF Uptake Quantification

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For EGF uptake assays, HCC1954 pLKO cells and Endo KD cells seeded on coverslips were incubated with HER2 affibody for 30 min on ice, then switched to media containing Texas Red-conjugated EGF (100 ng/ml, Molecular Probes) at 37 °C for 15 min or kept on ice prior to fixation (1% paraformaldehyde for 15 minutes), and counterstaining with 4′,6-diamidino-2-phenylindole (DAPI) (1:400). Image acquisition was performed on a Quorum WaveFX-X1 spinning disc (Quorum Technologies Inc.), and a LSM800 Airyscan super-resolution confocal microscope (Zeiss). The images were analyzed for the presence and co-localization of EGF and HER2 marked vesicles using Image-Pro Plus 6 (Media Cybernetics).

Baldassarre T., Truesdell P, & Craig A.W. (2017). Endophilin A2 promotes HER2 internalization and sensitivity to trastuzumab-based therapy in HER2-positive breast cancers. Breast Cancer Research : BCR, 19, 110.

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Quantifying Cell Migration and Invasion

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Scratch wound migration assays were performed on H1299 V and KD cells as previously described.^{10 (link)} For A549 cells, a 35 mm μ-Dish (Ibidi) was used with complete media containing EGF (50 ng/ml) for 9 hours. For live cell migration assays, H1299 V and KD1 cells were grown to confluence in either side of a 35 mm μ-Dish (Ibidi), serum starved overnight, and culture insert removed prior to imaging on a Quorum WaveFX-X1 spinning disc confocal microscope (Quorum Technologies Inc.). Images were captured every 15 minutes for 18 hours. Videos were created using MetaMorph Microscopy Automation & Image Analysis Software (Molecular Devices). The migration distance of individual cells was measured using Image J software with Manual Tracker and Click Forward add-ons. Transwell invasion assays were performed as previously described.^{10 (link)} A549 cells were treated with TGF-β1 (2 ng/ml) prior to these assays to enhance their invasiveness. After 48 hrs, DAPI-stained cells were imaged by epifluorescence microscopy (4 fields/insert) and scored using Image Pro Plus.

Truesdell P., Ahn J., Chander H., Meens J., Watt K., Yang X, & Craig A. (2014). CIP4 Promotes Lung Adenocarcinoma Metastasis and Is Associated with Poor Prognosis. Oncogene, 34(27), 3527-3535.

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Visualizing Myc B Effects on F-actin

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Immunofluorescence was used to visualize the effects of Myc B treatment on specific F-actin structures. Acid washed coverslips were coated with human fibronectin prior to seeding of SKOV3 cells, and treatment with DMSO or Myc B (25 nM) for 2 hours. Cells were then fixed with 4% PFA, permeabilized in 0.2% Triton X-100 followed by overnight incubation with anti-cortactin (1:200, Millipore ab#3852) antibody in a humidity chamber at 4 °C. Coverslips were then rinsed with PBS and incubated in the dark for 1 hour at RT with Alexa Fluor® 568-conjugated goat anti-rabbit IgG (1:2000, Invitrogen), TRITC-Phalloidin (1:200, Sigma), and DAPI (1:400, Sigma). Images were acquired using a Quorum WaveFX-X1 spinning disc confocal system (Quorum Technologies Inc., Guelph), and analyzed using Metamorph software.

Nersesian S., Williams R., Newsted D., Shah K., Young S., Evans P.A., Allingham J.S, & Craig A.W. (2018). Effects of Modulating Actin Dynamics on HER2 Cancer Cell Motility and Metastasis. Scientific Reports, 8, 17243.

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