Dulbecco’s modified Eagle medium (DMEM), foetal bovine serum (FBS) and antibiotics (penicillin and streptomycin) required for the cell cultures were obtained from GIBCO (Carlsbad, CA, USA). The cell culture plates were manufactured by Corning Inc. (Corning, NY, USA). The CCK-8 and DAPI working solution were purchased from Beyotime Biotechnology (Shanghai, China). l -glutamine, tunicamycin (TUNI), doxorubicin (DOX) and dimethylsulfoxide (DMSO) were purchased from Sigma (Saint Louis, MO, USA). The antibodies against XBP-1s and phospho-PERK were obtained from Santa Cruz Biotechnology, and the antibodies against PERK, ATF6, IRE1α, GRP78, CHOP/GADD153, ATF4 cleaved- caspase3, and β-actin, plus the Alexa Fluor 488-conjugated secondary antibodies, were obtained from Abcam (Cambridge, MA, USA).
Chop gadd153
Manufactured by Abcam
Sourced in United States
CHOP/GADD153 is a protein that functions as a transcription factor, regulating the expression of genes involved in cellular stress response pathways. It is an important component of the unfolded protein response (UPR), which is activated in response to the accumulation of misfolded proteins in the endoplasmic reticulum.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated secondary antibody, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Dapi working solution, by Beyotime (1 mentions)
Pureproteome magnetic bead, by Merck Group (1 mentions)
C ebp β, by Merck Group (1 mentions)
C ebpβ, by Santa Cruz Biotechnology (1 mentions)
β tubulin, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by GeneTex (1 mentions)
2 protocols using chop gadd153
1
Investigating Cellular Stress Responses
2
Protein and Tumor Immunoblotting Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein or tumor extracts (20 μg) were subjected to SDS-PAGE and probed with the following antibodies: C/EBP-β (directed against the common C-terminus of LIP and LAP, Santa Cruz Biotechnology Inc., Santa Cruz, CA), CHOP/GADD153 (Abcam, Cambridge, UK), TRB3 (Proteintech, Chicago, IL), caspase-3 (GeneTex, Hsinhu City, Taiwan), β-tubulin (Santa Cruz Biotechnology Inc.). To detect ubiquitinated C/EBP-β, 100 μg protein extracts were immunoprecipitated overnight with the anti-C/EBP-β antibody, using 25 μl of PureProteome Magnetic Beads (Millipore). Immunoprecipitated samples were then probed with an anti-mono/polyubiquitin antibody (Axxora). Blotting was followed by the peroxidase-conjugated secondary antibody. The membranes were washed with Tris-buffered saline/Tween 0.01% v/v and proteins were detected by enhanced chemiluminescence. Band density was calculated using ImageJ software (http://www.rsb.info.nih.gov/ij/).
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