A total of 5000 cells were seeded into 96-well plates with six technical replicates. The next day, the cells were treated with 2 μg/ml doxycycline after transfection. For drug sensitivity validation, cells were treated with 750 nM obatoclax (Beyotime, SC4381) and 10 μM triciribine (Beyotime, SF2721) for 24 h after 48 h of transfection. At the indicated time point, the mixed solution of 100 μl DMEM and 10 μl CCK8 (A311, Vazyme, china) was added into each well and the cells were further incubated at 37°C for 30–60 minutes. The absorbance was measured at 450 nm after a half-hour incubation at 37°C.
Triciribine
Manufactured by Beyotime
Sourced in China
Triciribine is an organic compound used as a research tool in scientific laboratories. It functions as an inhibitor of the Akt/PKB signaling pathway, which is involved in cellular processes such as metabolism, cell growth, and survival. Triciribine is commonly utilized in cell-based assays and studies to investigate the role of the Akt/PKB pathway in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Obatoclax, by Beyotime (1 mentions)
Cck 8, by Vazyme (1 mentions)
Ps6 ser235 236, by Cell Signaling Technology (1 mentions)
Hsp70, by Beyotime (1 mentions)
Lamp1, by Cell Signaling Technology (1 mentions)
Gapdh, by Bioss Antibodies (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
P mtor ser2448, by Wanlei (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Ly294002, by Beyotime (1 mentions)
Chloroquine, by Merck Group (1 mentions)
2 protocols using triciribine
1
Cell Viability Assay with Obatoclax and Triciribine
2
Exosome Isolation and Characterization from MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The supernatant was obtained from the MSC medium with or without GW4869 that were cultured for 3 days. The MSC medium with or without GW4869 that were cultured for 3 days was ultracentrifuged to get exosomes. The exosomes were collected and resuspended in sterile 1×PBS for future use.
LY294002 (20 μM), LY303511 (10 μM), Triciribine (10 μM), mTOR, HSP70, CD9, CD63, and ULK1 were purchased from Beyotime (Shanghai, China). 3-MA (10 mM) was purchased from Selleckchem (Houston, USA). GW4869 (10 μM) was purchased from Targetmol (Boston, USA). Chloroquine (20 μM) was purchased from Merck (New Jersey, USA). Dorsomorphin (10 μM) was purchased from Glpbio (California, USA). p-AMPK/AMPK, p-mTOR(ser2448) were purchased from Wanleibio (Shenyang, China). p-AKT/AKT1/2/3 were purchased from Abmart (Shanghai, China). Bax, Bcl-2, Caspase3, Lamp1, LC3B, Beclin1, p-S6 (ser235/236), and p62 were purchased from Cell Signaling Technology (Boston, USA). Ataxin-3 was purchased from Millipore (Massachusetts, USA). GAPDH and p-TFEB (ser142) was purchased from Bioss (Beijing, China). TFEB was purchased from Abcam (Massachusetts, USA). Detailed information is listed in the Supplementary Table.
LY294002 (20 μM), LY303511 (10 μM), Triciribine (10 μM), mTOR, HSP70, CD9, CD63, and ULK1 were purchased from Beyotime (Shanghai, China). 3-MA (10 mM) was purchased from Selleckchem (Houston, USA). GW4869 (10 μM) was purchased from Targetmol (Boston, USA). Chloroquine (20 μM) was purchased from Merck (New Jersey, USA). Dorsomorphin (10 μM) was purchased from Glpbio (California, USA). p-AMPK/AMPK, p-mTOR(ser2448) were purchased from Wanleibio (Shenyang, China). p-AKT/AKT1/2/3 were purchased from Abmart (Shanghai, China). Bax, Bcl-2, Caspase3, Lamp1, LC3B, Beclin1, p-S6 (ser235/236), and p62 were purchased from Cell Signaling Technology (Boston, USA). Ataxin-3 was purchased from Millipore (Massachusetts, USA). GAPDH and p-TFEB (ser142) was purchased from Bioss (Beijing, China). TFEB was purchased from Abcam (Massachusetts, USA). Detailed information is listed in the Supplementary Table.
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