Immunohistochemical analyses were performed by the Histology facility at the CRUK Beatson Institute using standard protocols on Bond Rx or Dako autostainers. Anti‐Ki‐67 (clone SP6; 1 : 100) was purchased from Abcam, and anti‐caspase 3 (clone Asp‐175; 1 : 500) was purchased from Cell Signaling. Quantitative analysis of positive staining was performed by counting cells in at least three high‐power fields of view (×40) per tumor by two independent researchers who were blinded to the sample group. Images were captured on an Axio Imager A2 Bio upright microscope (Zeiss, Cambridge, UK) using zenpro 2012 software (Zeiss).
Anti caspase 3 clone asp 175
Manufactured by Cell Signaling Technology
Anti‐caspase 3 (clone Asp‐175) is a lab equipment product that targets the caspase 3 protein. Caspase 3 is a key effector caspase involved in the execution phase of cell apoptosis. This product can be used to detect and study the caspase 3 protein.
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2 protocols using anti caspase 3 clone asp 175
1
Immunohistochemical Analysis of Tumor Samples
2
Quantitative Immunohistochemical Analysis
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Immunohistochemical analyses were performed by the Histology facility at the CRUK Beatson Institute using standard protocols on Bond Rx or Dako autostainers. Anti-Ki67 (clone SP6; 1:100) was purchased from Abcam and anti-Caspase 3 (clone Asp-175; 1:500) was purchased from Cell Signaling. Quantitative analysis of positive staining was performed by counting cells in at least 3 high-power fields of view (x40) per tumor by two independent researchers who were blinded to the sample group. Images were captured on an Axio Imager A2 Bio upright microscope (Zeiss) using ZenPro 2012 software (Zeiss).
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