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X tra slides

Manufactured by Leica
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Sourced in United Kingdom

X-tra slides are high-quality glass microscope slides designed for use in Leica's laboratory equipment. They provide a durable and consistent surface for specimen mounting and observation.

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Lab products found in correlation

α sma, by Agilent Technologies (1 mentions) Hematoxylin, by Merck Group (1 mentions) Ep1551y, by Abcam (1 mentions) Peroxidase anti rabbit igg h l, by Vector Laboratories (1 mentions) Vector dab peroxidase substrate kit, by Vector Laboratories (1 mentions) Vector red alkaline phosphatase substrate kit, by Vector Laboratories (1 mentions) Normal agarose, by Merck Group (1 mentions) Lmp agarose, by Merck Group (1 mentions)

Close spelling variants of this product

Surgipath X-tra Adhesive Microscope slides X-tra Adhesive Precleaned Micro Slides X-tra adhesive slides X-tra® adhesive glass slides Surgipath X‐tra Adhesive micro slides Surgipath® R X-Tra® R Microscope Slides

4 protocols using x tra slides

1

Bdellovibrio attachment assay protocol

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Attachment assays were performed according to Milner et al.20 (link) with slight modifications. HI and HI flp mutants were grown in PYE medium to an OD600 of 0.5, washed twice in DNB, and mixed with E. coli. The experiments were carried out at the final cell concentrations of 106 and of 107 ml−1. The Bdellovibrio - E. coli co-culture was incubated for recovery of 30 min in 28 °C, then concentrated by low speed centrifugation (4000 rpm for 10 min), the pellet was re-suspended in fresh DNB and re-incubated in 28 °C. 10 µl were taken at different time points, placed on positively charged polylysine-coated adhesive microscope slide (X-tra slides, Leica) and examined under phase contrast microscopy. As a negative control, E. coli was replaced with Bacillus subtilis, a gram-positive bacterium that Bdellovibrio does not attach to.
Avidan O., Petrenko M., Becker R., Beck S., Linscheid M., Pietrokovski S, & Jurkevitch E. (2017). Identification and Characterization of Differentially-Regulated Type IVb Pilin Genes Necessary for Predation in Obligate Bacterial Predators. Scientific Reports, 7, 1013.
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2

Immunohistochemical Staining of FFPE Tissues

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FFPE tissue blocks were sectioned serially at 5 µm and mounted onto Xtra Slides (Leica). After drying (37°C overnight), heat-induced antigen retrieval was carried out as previously described (Humphries et al. 2017) (link). Briefly, this was achieved by pressure-cooking in 10% antigen retrieval Access Revelation Solution -10× solution -at 125°C (Menarini Diagnostics, Wokingham, UK). CD68, CD8 and αSMA (all 1:100; Dako) were applied to slides and incubated for 30, 60 and 30 min, respectively. Novolink Polymer Detection System kit
Lees T., Cullinane A., Condon A., Shabaan A.M., Humphries M.P, & Speirs V. (2018). Characterising the adipose-inflammatory microenvironment in male breast cancer. Endocrine-related cancer, 25(7).
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3

Dual-Stain Immunohistochemistry Protocol

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Frozen tissue blocks were cut at 5 µm thickness and picked up on X-tra® Slides (Leica Biosystems). After air-drying for 30 min at room temperature, sections were immediately fixed with acetone at −20°C for 20 min. Slides were rehydrated in 1× PBS for 10 min. To quench endogenous peroxidase activity, sections were incubated with peroxidase reagent (3% H2O2 in 1× PBS) for 15 min and gently washed twice in 1× PBS for 5 min. Slides incubated with a primary antibody cocktail overnight at 2°C to 8°C. The cocktail contained both monoclonal mouse anti-human CD3, (Clone F7.2.38; 1:100, Dako) and anti-p16 ARC antibody (EP1551Y; 1:100, Abcam, ab51243). Upon finishing and rinsing, the secondary antibody cocktail [peroxidase anti-rabbit IgG (H+L) (Vector Laboratories, PI-1000) and alkaline phosphatase anti-mouse IgG (H+L) (Vector Laboratories, AP-2000)] was prepared and applied as recommended by manufacturer (Vector Laboratories) followed by the subsequent visualization of AP activity (Vector Red Alkaline Phosphatase Substrate Kit) (Vector Laboratories, SK-5100) and HRP activity (Vector DAB Peroxidase Substrate Kit) (Vector Laboratories, SK-4100). Stained tissues were mounted with hematoxylin (Sigma-Aldrich), dehydrated, covered and imaged via microscope.
Li Y., Shen Y., Hohensinner P., Ju J., Wen Z., Goodman S.B., Zhang H., Goronzy J.J, & Weyand C.M. (2016). Deficient activity of the nuclease MRE11A induces T cell aging and promotes arthritogenic effector functions in patients with rheumatoid arthritis. Immunity, 45(4), 903-916.
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4

Comet Assay for Evaluating CNOT1 Knockdown

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Comet slides (X-tra® Slides from Leica Biosystem, Milton Keynes, UK) were prepared by scratching with an engraving pen and then coating with 0.6% normal agarose (Sigmaaldrich, St. Louis, MI, USA) to a depth of 2–3 mm. Then, 5 × 105 cells, treated with control or CNOT1 siRNA for 72 h, were suspended in 1.2% LMP agarose (Sigmaaldrich, St. Louis, MI, USA) (each sample was in triplicate) and added to the slides. After electrophoresis, the slides were rinsed with ice-cold water and incubated in 1 M Tris HCl pH 7.0 for 30 min, rinsed again in ice-cold water, heated at 42 °C overnight and then placed at 4 °C. The slides were stained with 1 mL of SYBR Green in PBS and cover slips were applied. A total of 200 comets were viewed at 20× magnification, and the mean tail moment was calculated using Open Comet plugin ImageJ bundle (original macro from Herbert M. Geller, NIH, 1997, later development by Robert Bagnell, 2011, UNC-CH).
Hagkarim N.C., Hajkarim M.C., Suzuki T., Fujiwara T., Winkler G.S., Stewart G.S, & Grand R.J. (2023). Disruption of the Mammalian Ccr4–Not Complex Contributes to Transcription-Mediated Genome Instability. Cells, 12(14), 1868.
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