Overnight fasting blood samples from all participants were collected from the antecubital vein. Enzymatic methods were applied to measure total cholesterol, high-density lipoprotein cholesterol, and triglycerides levels (ADVIA 1800 Auto Analyzer; Siemens Medical Solutions, Malvern, PA, USA). Fasting blood glucose concentrations were measured using a colorimetry method (ADVIA 1800 Auto Analyzer; Siemens Medical Solutions). Serum insulin concentrations were measured in accordance with a radioimmunoassay (SR-300; Stratec, Birkenfeld, Germany). Hemoglobin A1c concentrations were measured by high performance liquid chromatography (Variant II TURBO, Bio-Rad, Hercules, CA, USA) according to the National Glycohemoglobin Standardization Program. C-reactive protein (CRP) concentrations were determined in accordance with a turbidimetric immunoassay (ADVIA 1800 Auto Analyzer; Siemens Medical Solutions).
Sr 300
Manufactured by Stratec
Sourced in Germany
The SR-300 is a high-performance analytical centrifuge designed for a variety of laboratory applications. It features a compact and durable design, and is capable of achieving high speeds and g-forces. The SR-300 is suitable for use in research, testing, and other laboratory settings, where precise separation and analysis of samples is required.
Automatically generated - may contain errors
6 protocols using sr 300
1
Metabolic biomarker assessment in fasting blood
2
Metabolic and Inflammatory Biomarkers
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Blood samples were collected from the antecubital vein
after over 8 hr on fasting. All analyses were performed at a single laboratory center (Seoul Clinical Laboratories R&D Center, Seoul, Korea). Fasting blood glucose and creatinine level was determined by a colorimetry method (ADVIA 1800; Siemens, Tarrytown, NY, USA). HbA1c was determined using high-performance liquid chromatography with a Variant II Turbo (Bio-Rad Laboratories, Hercules, CA, USA). Fasting insulin levels were determined by a radioimmunoassay with SR-300 apparatus (Stratec; Birkenfeld, Rhineland-Palatinate, Germany). HOMA-IR was used to evaluate IR: HOMA-IR=fasting glucose (mg/dL)×fasting insulin (µIU/mL)/405.[12 (link)] Total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and high-sensitivity C-reactive protein (hs-CRP) levels were assayed by enzymatic methods (ADVIA 1800; Siemens). Serum 25-hydroxy-vitamin D (25[OH]D) level was assessed by a chemiluminescence immunoassay (DiaSorin, Dietzenbach, Germany). The Chronic Kidney Disease-Epidemiology equation was used to calculate the estimated glomerular filtration rate.[13 (link)]
after over 8 hr on fasting. All analyses were performed at a single laboratory center (Seoul Clinical Laboratories R&D Center, Seoul, Korea). Fasting blood glucose and creatinine level was determined by a colorimetry method (ADVIA 1800; Siemens, Tarrytown, NY, USA). HbA1c was determined using high-performance liquid chromatography with a Variant II Turbo (Bio-Rad Laboratories, Hercules, CA, USA). Fasting insulin levels were determined by a radioimmunoassay with SR-300 apparatus (Stratec; Birkenfeld, Rhineland-Palatinate, Germany). HOMA-IR was used to evaluate IR: HOMA-IR=fasting glucose (mg/dL)×fasting insulin (µIU/mL)/405.[12 (link)] Total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and high-sensitivity C-reactive protein (hs-CRP) levels were assayed by enzymatic methods (ADVIA 1800; Siemens). Serum 25-hydroxy-vitamin D (25[OH]D) level was assessed by a chemiluminescence immunoassay (DiaSorin, Dietzenbach, Germany). The Chronic Kidney Disease-Epidemiology equation was used to calculate the estimated glomerular filtration rate.[13 (link)]
3
Metabolic Profile Assessment Protocol
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After at least 8 h of fasting, blood samples were collected from the antecubital vein in the morning. Fasting blood glucose levels were determined by a colorimetry method (ADVIA 1800; Siemens Medical Solutions, Pleasanton, CA, USA), and fasting insulin levels were determined using an immunoradiometric assay (SR-300; Stratec, Birkenfeld, Germany). Enzymatic methods were used to measure the total cholesterol, high-density cholesterol (HDL) and triglyceride levels (ADVIA 1800; Siemens Medical Solutions, Pleasanton, CA, USA). Low-density lipoprotein cholesterol levels were calculated using the Friedewald formula. 11 C-reactive protein (CRP) concentration was assessed with a turbidimetric immunoassay (ADVIA 1800, Wako, Japan). The homeostasis model assessment of insulin resistance (HOMA-IR) was used to evaluate insulin resistance: HOMA-IR = fasting glucose (mg dl À 1 ) × fasting insulin (μIU ml À 1 )/405. 12 Incident diabetes was defined by a fasting glucose level ⩾ 126 mg dl À 1 or an HbA1c ⩾ 6.5% at the follow-up examination and/ or a physician's diagnosis of diabetes during the follow-up period.
4
Metabolic Biomarker Profiling Protocol
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Blood samples from overnight fast participants were collected from the antecubital vein. Enzymatic methods were used to measure the levels of total cholesterol, high-density lipoprotein cholesterol, and triglycerides (ADVIA 1800 Auto Analyzer, Siemens medical Sol., Deerfield, IL, USA). Fasting blood glucose concentrations were measured using a colorimetry method (ADVIA 1800 Auto Analyzer, Siemens medical Sol., Deerfield, IL, USA). Serum insulin concentrations were measured in accordance with the instructions of a radioimmunoassay (SR-300, Stratec, Birkenfeld, Germany). Hemoglobin A1c (HbA1c) concentrations were measured with high performance liquid chromatography (Variant II TURBO, Bio-Rad, Berkeley, California, USA) according to the National Glycohemoglobin Standardization Program. Uric acid concentrations were measured using a colorimetry method (ADVIA 1800 Auto Analyzer, Siemens medical Sol., Deerfield, IL, USA). C-reactive protein (CRP) concentrations were determined using a turbidimetric immunoassay (ADVIA 1800 Auto Analyzer, Siemens medical Sol., Deerfield, IL, USA).
5
Acromegaly Demographic and Metabolic Profiles
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Demographic data from 30 patients with acromegaly were reviewed. Demographic parameters (age, gender, height, weight, and systolic blood pressure [SBP] and diastolic blood pressure [DBP]) were collected from the medical charts when they visited our hospital at first visit. Body mass index (BMI, kg/m2) was also calculated based on their height and weight.
Blood samples were collected in the morning after overnight fasting. Fasting plasma glucose (FPG) was measured by the hexokinase method (Roche Diagnostics, Basel, Switzerland). Low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were measured using a selective enzymatic protection assay. Triglycerides were measured by enzymatic assay without a glycerol blank using a Hitachi Molecular Analytics D2400 apparatus (Roche, Tokyo, Japan). Serum GH and IGF-1 were measured by radioimmunoassay using SR-300 (STRATEC, Birkenfeld, Germany).
Blood samples were collected in the morning after overnight fasting. Fasting plasma glucose (FPG) was measured by the hexokinase method (Roche Diagnostics, Basel, Switzerland). Low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were measured using a selective enzymatic protection assay. Triglycerides were measured by enzymatic assay without a glycerol blank using a Hitachi Molecular Analytics D2400 apparatus (Roche, Tokyo, Japan). Serum GH and IGF-1 were measured by radioimmunoassay using SR-300 (STRATEC, Birkenfeld, Germany).
6
Thyroid Function and Celiac Disease Biomarkers
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ATPO (normal range, 0–78 U/ml), TSH (normal range 0.27–3.5 mU/ml) and FT4 (normal range 10–22 pmol/l) were measured using open analyzer system on a radioactive basis for radioimmunoassays SR300 (Stratec Biomedical Systems AG (Germany)).
Thyroid function was evaluated as hypothyroidism, subclinical hypothyroidism, hyperthyroidism and subclinical hyperthyroidism. Subclinical hypothyroidism was diagnosed when FT4 levels were within normal reference laboratory range but TSH levels were elevated. Subclinical hyperthyroidism was defined as TSH level below 0.27 mU/ml and FT4 within normal range.
Tissue transglutaminase IgA antibodies (tTG-A) were measured by enzyme immunoassay (ELISA) kits, using Gemini analyzer (Stratec Biomedical Systems AG (Germany)). Concentrations >18 U/ml for tTG-A were considered positive, - between 12 and 18 U/ml – borderline and concentrations <12 U/ml - normal.
Thyroid function was evaluated as hypothyroidism, subclinical hypothyroidism, hyperthyroidism and subclinical hyperthyroidism. Subclinical hypothyroidism was diagnosed when FT4 levels were within normal reference laboratory range but TSH levels were elevated. Subclinical hyperthyroidism was defined as TSH level below 0.27 mU/ml and FT4 within normal range.
Tissue transglutaminase IgA antibodies (tTG-A) were measured by enzyme immunoassay (ELISA) kits, using Gemini analyzer (Stratec Biomedical Systems AG (Germany)). Concentrations >18 U/ml for tTG-A were considered positive, - between 12 and 18 U/ml – borderline and concentrations <12 U/ml - normal.
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