U2910 ultraviolet spectrophotometer

Optical rotations were measured at room temperature on an Anton Paar polarimeter (Anton Paar, Graz, Austria). UV spectra were recorded on a Hitachi U2910 ultraviolet spectrophotometer. ECD measurements were performed using a JASCO J-810 spectropolarimeter. IR spectra were recorded on a Nicolet 6700 FT-IR spectrometer. ¹H and ¹³C, DEPT 90, DEPT 135, HSQC, HMBC, NOESY, and COSY NMR spectra were recorded at room temperature on a Bruker Avance II 400, Bruker Avance III HD 700, or a Bruker Avance III HD 800 MHz NMR spectrometer. ESIMS or HRESIMS data were collected on a Bruker Maxis 4G Q-TOF mass spectrometer in the positive-ion mode. Column chromatography was conducted using silica gel (65 × 250 or 230 × 400 mesh, Sorbent Technologies, Atlanta, GA, USA). Analytical TLC was performed on precoated silica gel 60 F254 plates (Sorbent Technologies, Atlanta, GA, USA). Sephadex LH-20 was purchased from Amersham Biosciences, Uppsala, Sweden. For visualization of TLC plates, H₂SO₄ was used as a spray reagent. All procedures were carried out using solvents purchased from commercial sources and employed without further purification. (+)-Digoxin, paclitaxel, and other reagents for chemical synthesis were purchased from Sigma-Aldrich (St. Louis, MO, USA) (purity ≥ 98%).

Optical rotations were measured at room temperature on an Anton Paar polarimeter (Anton Paar, Graz, Austria). UV spectra were recorded on a Hitachi U2910 ultraviolet spectrophotometer. ECD measurements were performed using a JASCO J-810 spectropolarimeter. IR spectra were recorded on a Nicolet 6700 FT-IR spectrometer. ¹H and ¹³C, DEPT 90, DEPT 135, HSQC, HMBC, NOESY, and COSY NMR spectra were recorded at room temperature on a Bruker Avance II 400, a Bruker Avance III HD 700, or a Bruker Avance III HD 800 MHz NMR spectrometer. ESIMS or HRESIMS data were collected on a Bruker Maxis 4G Q-TOF mass spectrometer in the positive-ion mode. Column chromatography was conducted using silica gel (65 × 250 or 230 × 400 mesh, Sorbent Technologies, Atlanta, GA, USA). Analytical thin-layer chromatography (TLC) was performed on precoated silica gel 60 F254 plates (Sorbent Technologies, Atlanta, GA, USA). Sephadex LH-20 was purchased from Amersham Biosciences, Uppsala, Sweden. For visualization of TLC plates, H₂SO₄ was used as a spray reagent. All procedures were carried out using solvents purchased from commercial sources and employed without further purification. Paclitaxel and reagents for chemical synthesis were purchased from Sigma-Aldrich (St. Louis, MO, USA) (purity ≥ 98%).

Optical rotations were measured at room temperature on a Perkin-Elmer model 343 polarimeter with a path length of 10 mm. UV spectra were recorded on a Hitachi U2910 ultraviolet spectrophotometer. ECD measurements were performed using a JASCO J-810 spectropolarimeter. IR spectra were recorded on a Nicolet 6700 FT-IR spectrometer. ¹H and ¹³C, DEPT 90, DEPT 135, HSQC, HMBC, NOESY, and COSY NMR spectra were recorded at room temperature on a Bruker Avance DRX 400, Bruker Avance II 400, Bruker Avance III HD 700, or a Bruker Avance III HD 800 MHz NMR spectrometer. ESIMS and HRESIMS data were collected on a Bruker Maxis 4G Q-TOF mass spectrometer in the positive-ion mode. Column chromatography was conducted using silica gel (65 × 250 or 230 × 400 mesh, Sorbent Technologies, Atlanta, GA, USA). Analytical thin-layer chromatography (TLC) was performed on precoated silica gel 60 F254 plates (Sorbent Technologies, Atlanta, GA, USA). Sephadex LH-20 was purchased from Amersham Biosciences, Uppsala, Sweden. For visualization of TLC plates, H₂SO₄ was used as spray reagent. All procedures were carried out using solvents purchased from commercial sources and employed without further purification. Digoxin, paclitaxel, and other reagents for chemical synthesis were purchased from Sigma-Aldrich (St. Louis, MO, USA) (purity ≥ 98%).