Anti tead1

Cells were harvested and lysed with RIPA buffer. Proteins were separated by electrophoresis on SDS-polyacrylamide gel electrophoresis (PAGE) and electro-transferred to PVDF membrane. Membranes were washed with TBST and incubated with primary antibodies for 2 h. And then the membranes were washed three times with TBST and incubated with second antibodies for 2 h, after being washed three times with TBST, the membranes were probed with ECL system (Cytiva, Cat. No. RPN2105). The antibodies used in this study were listed here: Anti- YAP (Santa Cruz, Cat. No. sc-101199); Anti- ERα (Cell Signaling Technology, Cat. No. sc-D8H8); Anti-TEAD4 (Santa Cruz, Cat. No. sc-390578); Anti-TEAD1(BD Transduction Laboratories, Cat. No. 610922); Anti-HA (COVANCE, Cat. No. MMS-101R); Anti-Myc (Abcam, Cat. No. ab32); Anti-Myc (Abcam, Cat. No. Ab9106); Anti-Flag (Abcam, Cat. No. Ab49763,), Peroxidase-Conjugated AffiniPure Goat Anti-Mouse IgG (Jackson ImmunoResearch Code,115-035-003), or Goat Anti-Rabbit IgG (Jackson ImmunoResearch, Code,111-035-144). Chemiluminescent signals were visualized with ECL system (Cytiva, Cat. No. RPN2105).