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Human cxcl1 gro alpha elisa kit

Manufactured by R&D Systems

The Human CXCL1/GRO alpha ELISA Kit is an enzyme-linked immunosorbent assay designed for the quantitative detection of human CXCL1/GRO alpha in biological samples such as cell culture supernatants, serum, and plasma.

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2 protocols using human cxcl1 gro alpha elisa kit

1

CXCL1 Quantification via ELISA

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The CXCL1 concentrations in the cell supernatants were measured via ELISA according to the manufacturer’s instructions. The following ELISA kits were used in this study: mouse CXCL1 ELISA kit (R&D Systems) and human CXCL1/GRO alpha ELISA kit (R&D Systems).
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2

Cytokine Profiling in Cell Culture

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Cells were cultured for 24 or 72 h. The cytokine concentrations in the cell supernatants were measured via ELISAs or human cytokine array, according to the manufacturer’s instructions. Infinite® M1000 Pro (Tecan) was used to measure absorbance. The following ELISA kits were used in this study: mouse MCP-1/CCL2 ELISA kit (88-7391-22, Invitrogen), mouse CXCL1/KC ELISA kit (DY453, R&D Systems), human CXCL1/GRO alpha ELISA kit (DY275, R&D Systems), and human CXCL8 ELISA kit (88-8086-88, Invitrogen). The human cytokine array (ARY005) was from R&D Systems (Minneapolis, MN).
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