RML12 Aedes albopictus cells with and Wolbachia-free wMel was grown at 24°C in Schneider’s insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Corning), 1% each of L-Glutamine (Corning), non-essential amino acids (Corning), and penicillin-streptomycin-antimycotic (Corning). C7/10 Aedes albopictus cells with and Wolbachia-free were grown at 27°C under 5% ambient CO2 in 1X Minimal Essential Medium (Corning) supplemented with 5% heat-inactivated fetal bovine serum (Corning), 1% each of L-Glutamine (Corning), non-essential amino acids (Corning) and penicillin-streptomycin-antimycotic (Corning). Vertebrate baby hamster kidney fibroblast or BHK-21 cells were grown at 37°C under 5% ambient CO2 in 1X Minimal Essential Medium (Corning) supplemented with 10% heat-inactivated fetal bovine serum (Corning), 1% each of L-Glutamine (Corning), non-essential amino acids (Corning) and penicillin-streptomycin-antimycotic (Corning). JW18 Drosophila melanogaster cells with and without Wolbachia wMel were grown at 24°C in Shield and Sang M3 insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum, 1% each of L-Glutamine (Corning), non-essential amino acids (Corning), and penicillin-streptomycin-antimycotic (Corning).
Shield and sang m3 insect media
Manufactured by Merck Group
Shield and Sang M3 are insect cell culture media manufactured by Merck Group. Shield is a serum-free medium designed for the growth and maintenance of insect cell lines, while Sang M3 is a formulated medium specifically for the culture of Spodoptera frugiperda insect cells. Both products provide the necessary nutrients and growth factors required to support the in vitro propagation of insect cells.
Automatically generated - may contain errors
Lab products found in correlation
Minimal essential medium, by Corning (1 mentions)
Penicillin streptomycin antimycotic, by Corning (1 mentions)
Schneider s insect media, by Merck Group (1 mentions)
L glutamine, by Corning (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Non essential amino acids (neaa), by Corning (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using shield and sang m3 insect media
1
Cell culture conditions for Aedes, Drosophila, and BHK
2
Measuring Protein Synthesis in Drosophila Wing Discs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Third instar wandering larvae (day 7 AEL) were inverted in PBS, and the intestine and fat body were removed. The tissues were transferred to a solution of 5 μg/ml of puromycin (Thermo Fisher Scientific #A1113803) in Shield and Sang M3 insect media (Sigma-Aldrich #S8398) containing 10% FBS (Gibco, Life Technologies) for 20 min. Samples were then fixed in 4% PFA in PBS-T for 40 min and processed for immunostaining as described above. Regions of interests were outlined based on the nub>mRFP signal and the mean gray values of puromycin antibody signal measured. The mean gray values of the hinge and notum regions of the WDs were used to calculate the fluorescence signal intensity ratio.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!