Single-cell suspensions in FACS Buffer (HBSS [ThermoFisher 14170161, Ca/Mg-free] supplemented with 2% FCS) were pre-incubated with Fc-Block (BD 564220) before staining for surface antigens. The following antibodies were used to identify basal cells via flow cytometry: FITC anti-human THY1 (Biolegend, clone 5E10), Brilliant Violet 421 anti-human CD45 (Biolegend, clone HI30), Brilliant Violet 650 anti-human EPCAM (Biolegend, clone 9C4), APC/Cy7 anti-human ITGA6 (Biolegend, clone GoH3), PE/Cy7 anti-human NGFR (Biolegend, clone ME20.4), APC anti-human PDPN (Biolegend, clone NC-08). Cells were stained for 30 minutes on ice in FACS buffer and then washed for immediate sorting. Cells were sorted on a BD FACSAria Fusion cell sorter using BD FACSDiva software. Up to 10,000 Basal cells were sorted into 100 μL BAM banker (Wako chemicals) for Omni-ATAC-seq and cooled to −80°C using a “Mr. Frosty” freezing container (Thermo scientific). Samples were stored at −80°C until thawed for Omni-ATAC-seq. 1,000 Basal cells for bulk RNAseq were sorted directly into 5 μL TCL buffer (Qiagen). FlowJo v10 by TreeStar was used to generate plots.
Fitc anti human thy1
Manufactured by BioLegend
The FITC anti-human THY1 is a lab equipment product that can be used to detect the presence of the THY1 antigen in human samples. THY1, also known as CD90, is a cell surface glycoprotein expressed on various cell types. The FITC (Fluorescein Isothiocyanate) conjugate allows for the visualization and identification of THY1-positive cells using flow cytometry or other fluorescence-based detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Bambanker, by Fujifilm (2 mentions)
Facsaria fusion cell sorter, by BD (2 mentions)
Brilliant violet 421 anti human cd45, by BioLegend (2 mentions)
Brilliant violet 650 anti human epcam, by BioLegend (2 mentions)
Apc cy7 anti human itga6, by BioLegend (2 mentions)
Pe cy7 anti human ngfr, by BioLegend (2 mentions)
Apc anti human pdpn, by BioLegend (2 mentions)
Mr frosty freezing container, by Thermo Fisher Scientific (2 mentions)
Tcl buffer, by Qiagen (2 mentions)
Fc block, by BD (2 mentions)
2 protocols using fitc anti human thy1
1
Basal Cell Isolation and Profiling
2
Basal Cell Isolation and Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-cell suspensions in FACS Buffer (HBSS [ThermoFisher 14170161, Ca/Mg-free] supplemented with 2% FCS) were pre-incubated with Fc-Block (BD 564220) before staining for surface antigens. The following antibodies were used to identify basal cells via flow cytometry: FITC anti-human THY1 (Biolegend, clone 5E10), Brilliant Violet 421 anti-human CD45 (Biolegend, clone HI30), Brilliant Violet 650 anti-human EPCAM (Biolegend, clone 9C4), APC/Cy7 anti-human ITGA6 (Biolegend, clone GoH3), PE/Cy7 anti-human NGFR (Biolegend, clone ME20.4), APC anti-human PDPN (Biolegend, clone NC-08). Cells were stained for 30 minutes on ice in FACS buffer and then washed for immediate sorting. Cells were sorted on a BD FACSAria Fusion cell sorter using BD FACSDiva software. Up to 10,000 Basal cells were sorted into 100 μL BAM banker (Wako chemicals) for Omni-ATAC-seq and cooled to −80°C using a “Mr. Frosty” freezing container (Thermo scientific). Samples were stored at −80°C until thawed for Omni-ATAC-seq. 1,000 Basal cells for bulk RNAseq were sorted directly into 5 μL TCL buffer (Qiagen). FlowJo v10 by TreeStar was used to generate plots.
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