Transient transfections were performed using TransIT-LT1 Transfection Reagent (731-0029; VWR) for DNA plasmids and Lipofectamine RNAiMax (Thermo Fisher Scientific) for siRNAs according to the manufacturer’s protocol. 15–25 nM Dharmacon siRNAs (Horizon Discovery) and 5–10 nM Ambion siRNAs (Thermo Fisher Scientific) were used for reverse siRNA transfection (Supplementary file 2 ).
Dharmacon sirnas
Manufactured by Horizon Discovery
Dharmacon siRNAs are short, double-stranded RNA molecules designed to silence specific genes by targeting and degrading their mRNA. They are used in RNA interference (RNAi) experiments to study gene function and target validation.
Automatically generated - may contain errors
2 protocols using dharmacon sirnas
1
Transient Transfection of DNA and siRNA
2
Transient Transfection Protocols
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Transient transfections were performed using TransIT-LT1 Transfection Reagent (731-0029; VWR) for DNA plasmids and Lipofectamine RNAiMax (Thermo Fisher Scientific) for siRNAs according to the manufacturer's protocol. 15-25nM Dharmacon siRNAs (Horizon discovery) and 5-10nM Ambion siRNAs (Thermo Fisher) were used for reverse siRNA transfection (Table S2).
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