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2 protocols using activated neutral alumina

1

Synthesis and Characterization of Biomimetic Polymers

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Calcium chloride (CaCl2), magnesium sulfate (MgSO4), sodium hydrogen carbonate (NaHCO3), sodium chloride (NaCl), triethylamine (TEA) and hydrochloric acid (HCl, 1 M) were purchased from Fisher Scientific and used as received. Ethylene glycol (99.8%), anhydrous tetrahydrofuran (THF, 99%), 1-dodecanol (99%), α-bromoisobutyl bromide (98%), anisole (99%), oligo(Ethylene glycol methacrylate) (OEGMA, Mn = 300 g mol−1, 98%), Ethylene glycol dimethacylate (EGDMA), 2,2′-bipyridyl (bpy), copper(i) chloride (CuCl, 97%), activated neutral alumina, basic alumina, porcine mucin, bovine serum albumin (BSA, >98%), cholesterol, linoleic acid, phosphatidylcholine and polysorbate 80 were purchased from Sigma Aldrich and used as received. Potassium ethyl xanthogenate (96%), 2-bromoacetic acid, 4-(dimethylamino) pyridine (DMAP, >99%), N,N′-dicyclohexylcarbodiimide (DCC, >99%) and 2-bromoacetic acid were all purchased from Alfa Aesar and used as received. 4-(Dimethylamino)pyridinium-4-toluene sulfonate (DPTS) was synthesised as previously reported.25 (link) Polyacrylic acid (Carbopol 940) was purchased from Lubrizol. All solvents, unless stated otherwise, were reagent grade, purchased from Fisher Chemicals and used as received.
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2

Ruthenium-Catalyzed Reactions via Schlenk Line

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Reactions were carried out using standard Schlenk line techniques under nitrogen unless otherwise noted. Column chromatography was performed using 60 Å pore size 230–400 mesh silica gel (Sorbent Technologies) and 58 Å pore size activated neutral alumina (Sigma-Aldrich). All solvents used such as ethylene glycol, dichloromethane and hexane used were reagent grade. Tetrahy-drofuran (THF) was distilled from sodium benzophenone ketyl. Acetone and ethylene glycol were purchased from Fischer Scientific and VWR International, respectively. Ruthenium dodecacarbonyl, triphenylphosphine and dimethylphenylphosphine (Strem Chemicals) 2,2′-bipyridine, 4,4′-dimethyl-2,2′ebipyridine 2-phenylpyridine, 2-(p-tolyl)pyridine (Sigma-Aldrich) were used without further purifications. N,N-Diisopropylethylamine (DIPEA) was purchased from MP Biomedical LLC and Rhodamine-B needed for measurement of quantum yield was purchased from Sigma-Aldrich. The starting complexes K+[Ru(CO)3(TFA)3]− and Ru [(CO)2(TFA)2(PPh3)2] were synthesized according to literature procedures [1 ].
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