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Gpc software

Manufactured by Waters Corporation
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The GPC software from Waters Corporation is a comprehensive solution for Gel Permeation Chromatography (GPC) analysis. It provides the core functionality for data acquisition, processing, and reporting of GPC experiments.

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Lab products found in correlation

Alliance e2695 hplc system, by Waters Corporation (2 mentions) Tskgel g2000swxl, by Tosoh (2 mentions) Hp1100, by Agilent Technologies (1 mentions)

4 protocols using gpc software

1

GPC-Based Glycan Unit Allocation

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Waters GPC
software with a cubic spline fit was used to allocate
GU values to peaks. 2-AB-labeled glucose homopolymer (Ludger product
CAB-GHP-30, 2-AB glucose homopolymer ladder) was used as a system
suitability standard as well as an external calibration standard for
GU allocation (the system is deemed to be within specifications if
the peak width at half height for GU10 is less than 0.4 min).
Song K., Moon D.B., Kim N.Y, & Shin Y.K. (2020). Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β). ACS Omega, 5(12), 6619-6627.
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2

Molecular Weight Distribution Analysis of Deamidated Wheat Gluten Hydrolysate

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Molecular weight distribution profiles of the deamidated wheat gluten hydrolysate were determined by size-exclusion HPLC (Agilent HP1100, USA) with a TSKgel 2000 SWXL (300 mm × 7.8 mm) column. The temperature was 30°C. The mobile phase consisted of acetonitrile (40), water (60) and trifluoroacetic acid (0.1). The flow rate of the mobile phase was 0.5 ml min−1. The absorbance was monitored with UV detection at 220 nm.
A solution of 100 mg of sample diluted to 10 ml in the mobile phase was treated ultrasonically for 5 min to dissolve and centrifuged (Velocity 14R, Dynamic, Austria) at 10 000 r.p.m. After centrifugation and microfiltration, the sample was injected to be analysed. A mixture of four-protein standards containing cytochrome C (12 384 Da), bacillus enzyme (1422 Da), acetic acid–acetic acid–tyrosine–arginine (451 Da), acetic acid–acetic acid–acetic acid (189 Da) was taken to calibrate the column and make the standard curve. The plot of logMW against elution time was constructed, and the molecular weight distribution for each hydrolysate was then calculated according to the plot. Waters GPC software was used to analyse the chromatographic data.
Wang S., Meng D., Wang S., Zhang Z., Yang R, & Zhao W. (2018). Modification of wheat gluten for improvement of binding capacity with keratin in hair. Royal Society Open Science, 5(2), 171216.
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3

Determining Polycyclic Aromatic Hydrocarbon Molecular Weights

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The MW distributions of PCHs were determined by high-performance gel-ltration chromatography (HPLC) using a TSK gel G2000 SWXL (300 mm × 7.8, TOSOH, Tokyo, Japan) (Eric et al. 2013) (link). HPLC analysis was performed on a Waters e2695 Alliance HPLC system (Water Co., Milford, MA, USA), which was equipped with a 2487 UV detector and Empower workstation. The ow rate was set at 0.5 mL/min with the mobile phase consisting of acetonitrile/water/tri uoroacetic acid (45/55/0.1, v/v/v). Sample (10.0 μL) was injected into the system, and the column was maintained at a temperature of 30 ℃. The following standards: cytochrome C (12,500 Da), aprotinin (6,500 Da), bacitracin (1,450 Da), Gly-Gly-Tyr-Arg (451 Da), and Gly-Gly-Gly (189 Da) were used to build the calibration curve used to evaluate the MW of the sample. UV absorbance was recorded at 200 nm, and data were processed with gel-permeation chromatography (GPC) software (Waters Co., Milford, US).
Zhang W., Shi K., Han Y., Wang J., Yang C., Xu X, & Li B. (2022). Characterization of Pleurotus citrinopileatus hydrolysates obtained from Actinomucor elegans proteases compared with that by commercial proteases. Journal of food science, 87(9).
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4

Determining Polycyclic Aromatic Hydrocarbon Molecular Weights

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The MW distributions of PCHs were determined by high-performance gel-ltration chromatography (HPLC) using a TSK gel G2000 SWXL (300 mm × 7.8, TOSOH, Tokyo, Japan) (Eric et al. 2013) (link). HPLC analysis was performed on a Waters e2695 Alliance HPLC system (Water Co., Milford, MA, USA), which was equipped with a 2487 UV detector and Empower workstation. The ow rate was set at 0.5 mL/min with the mobile phase consisting of acetonitrile/water/tri uoroacetic acid (45/55/0.1, v/v/v). Sample (10.0 μL) was injected into the system, and the column was maintained at a temperature of 30 ℃. The following standards: cytochrome C (12,500 Da), aprotinin (6,500 Da), bacitracin (1,450 Da), Gly-Gly-Tyr-Arg (451 Da), and Gly-Gly-Gly (189 Da) were used to build the calibration curve used to evaluate the MW of the sample. UV absorbance was recorded at 200 nm, and data were processed with gel-permeation chromatography (GPC) software (Waters Co., Milford, US).
Zhang W., Shi K., Han Y., Wang J., Yang C., Xu X., & Li B. (2022). Characterization of Pleurotus Citrinopileatus Hydrolysates Obtained from Actinomucor Elegans Proteases Compared with That by Commercial Proteases.
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