Delta 9 tetrahydrocannabinol

Delta-9-tetrahydrocannabinol (THC), 11-nor-delta-8-tetrahydrocannabinol-9-carboxylic acid (THC-COOH), 11-hydroxy-Delta-9-tetrahydrocannabinol (11-OH-THC), 11-nor-Delta-9-tetrahydrocannabinol-9-carboxylic-acid-glucuronide (THC-COOH-gluc), cannabidiol (CBD), cannabigerol (CBG) and Delta-9-tetrahydrocannabinol-D3 (THC-D3) were purchased from Cerilliant (Milan, Italy); 11-nor-delta-9tetrahydrocannabinol-9-carboxylic-acid-D3 (THC-COOH-D3) was purchased from Lipomed AG (Arlesheim, Switzerland); LC-MS grade formic acid, methanol, acetonitrile, ethyl acetate, diethyl ether and isopropanol were obtained from Carlo Erba SRL (Milan, Italy); water was purified by filtering deionized water on a Milli-Q filtration system from Merck Millipore (Milan, Italy). Cards for DUS (four-spot cards, Whatman 903TM) were purchased from Sigma-Aldrich (Milan, Italy).

Eleven cannabinoids, namely cannabidivann (CBDV), tetrahydrocannabivarin (THCV), cannabidiol (CBD) with 99.66% purity (Lot: FE08071702), cannabigerol (CBG) with 98.98% purity (Lot: FE06241604), cannabidiolic acid (CBDA) with 98.3% purity (Lot: FE12011601), cannabigerolic acid (CBGA), cannabinol (CBN) with 99.37% purity (Lot: FE06131701), delta 9-tetrahydrocannabinol (Δ9-THC) with 97.66% purity (Lot: FE1041701), delta 8-tetrahydrocannabinol (Δ8-THC), cannabichomene (CBC) with 97.60% purity (Lot: FE10011502), and delta 9-Tetrahydrocannabinol acid (THCA-A) with 99.18% purity (Lot: FE12121601) stock solution with the concentration of 1000 µg/mL in acetonitrile or methanol (acid forms) as reference standards were purchased from Cerilliant, a Sigma Aldrich company (Kinesis Australia Pty Ltd., Redland Bay, QLD, and Novachem Pty Lt, Victoria, Australia). All other solvents (methanol, acetonitrile, and phosphoric acid) were purchased from Merck.