Sr apo tirf 100x 1.49 na

Laser scanning confocal imaging was conducted using a Nikon A1 Microscope equipped with 405, 488, 561, and 645 nm LASERs, Plan Apo 60X/1.4NA, and Plan Apo 25X/1.05 NA silicon (SIL) immersion objectives. Live-cell imaging was performed on a Nikon Ti2 inverted light microscope equipped with a Yokogawa CSU-X1 spinning disk head, equipped with 488 nm, 561 nm, and 647 nm excitation LASERs, a 405 nm photo-stimulation LASER directed by a Bruker mini-scanner to enable targeted photobleaching, a 100X Apo TIRF 100x/1.45 NA objective, and either a Hamamatsu Fusion BT or Photo-metrics Prime 95B sCMOS camera. Cells were maintained in a stage top incubator at 37°C with 5% CO2 (Tokai Hit). Super-resolution imaging was performed using a Nikon Structured Illumination Microscope (N-SIM) equipped with 405, 488, 561 and 640 nm LASERs, an SR Apo TIRF 100X/1.49 NA objective, and an Andor iXon Ultra DU-897 EMCCD camera. Images were reconstructed using Nikon Elements software. For imaging in all microscope modalities, imaging acquisition parameters were matched between samples during image acquisition. All images were denoised and deconvolved in Nikon Elements. As filopodia extremely thin structures, LUTs were optimized to facilitate visualization in figures.