Cytokine production (IL-4, IL-5, IL-9, IL-10, IL-13 and IFN-γ) from cell supernatant cultures (moDCs:CD14− PBMCs) were collected (100 µL) after 7 days. The cytokine production was determined using a human ProcartaPlex Multiplex Immunoassays kit (Thermo Fisher Scientific) following the manufacturer’s instructions. Briefly, the samples (50 µL) and standards were incubated (overnight at 4 °C) with the magnetic bead mix in a 96-well plate. After that, the biotinylated antibody was added and incubated for 300 min at RT, and following that, streptavidin was added and incubated for 300 min at RT. After each incubation, washing steps were performed. Finally, the plate was prepared with a reading buffer to be detected using Bio-Plex 200 (Bio-Rad, Hercules, CA, USA) [16 (link),25 (link)]. Data were analyzed using Bio-Plex Data Analysis Software (Bio-Rad Bio-Rad Laboratories, Inc, Hercules, CA, USA). The results were expressed as the fold change for the concentration (pg/mL) of each cytokine (cytokine production from supernatant of stimulated cell cocultures/cytokine production from supernatant of unstimulated cell cocultures).
Procartaplex multiplex immunoassays kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ProcartaPlex Multiplex Immunoassays kit is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to facilitate the simultaneous detection and quantification of multiple analytes in a single sample. The kit utilizes magnetic beads coated with specific antibodies to capture and measure the levels of various proteins, cytokines, or other biomolecules in a sample.
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Lab products found in correlation
3 protocols using procartaplex multiplex immunoassays kit
1
Multiplex Cytokine Quantification from moDCs-CD14- PBMCs
2
Cytokine Profiling of T-cell Interactions
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Cytokine production (IL-4, IL-5, IL-9, IL-10, IL-13, IFNγ, and IL-27) from supernatant cell cultures (T-cells alone, ILC2-T-cells contact or with Transwell) collected after 7 days was determined with a human ProcartaPlex Multiplex Immunoassays kit (Thermo Fisher Scientific), following the manufacturer’s indications, and analyzed in Bio-Plex 200 using Bio-Plex Data Analysis Software (Bio-Rad, Hercules, CA, USA). The results were presented as concentration (pg/mL) of each cytokine.
3
Cytokine Production Assessment of TLR-Pp3 T-Cells
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To determine cytokine production
from Pp3 or the different combination of TLR-Pp3-specific T-lymphocytes,
the supernatants from moDCs (48 h of culture) and the lymphocyte cultures
(6 days) were collected, and cytokine production (IL-4, IL-5, IL-10,
and IFNγ) was determined with a human ProcartaPlex Multiplex
Immunoassays kit (Thermo Fisher Scientific), following the manufacturer’s
indications and detected in a Bio-Plex 200 (Bio-Rad, Hercules, CA).
Data were analyzed using Bio-Plex Data Analysis Software (Bio-Rad,
Hercules, CA).
from Pp3 or the different combination of TLR-Pp3-specific T-lymphocytes,
the supernatants from moDCs (48 h of culture) and the lymphocyte cultures
(6 days) were collected, and cytokine production (IL-4, IL-5, IL-10,
and IFNγ) was determined with a human ProcartaPlex Multiplex
Immunoassays kit (Thermo Fisher Scientific), following the manufacturer’s
indications and detected in a Bio-Plex 200 (Bio-Rad, Hercules, CA).
Data were analyzed using Bio-Plex Data Analysis Software (Bio-Rad,
Hercules, CA).
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