The proliferation ability of the cells was detected by Cell-Light EdU Apollo643 in vitro kit (RIB BIO, China) according to the instructions of the manufacturer. In brief, the cells were incubated with solution A for 20–30 min at 37°C, then fixed with 4% paraformaldehyde in PBS for 15 min at room temperature, and sequentially incubated with solution Apollo643 for 20 min and Hoechst33342 for 5 min. The images were captured with the fluorescent phase-contrast microscope (Nikon).
Fluorescent phase contrast microscope
Manufactured by Nikon
The Fluorescent phase-contrast microscope is a laboratory instrument designed to observe and analyze samples using a combination of fluorescent and phase-contrast imaging techniques. It allows users to visualize and study a wide range of specimens, including live cells, tissues, and other biological samples.
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Lab products found in correlation
2 protocols using fluorescent phase contrast microscope
1
Cell Proliferation Detection with EdU
2
Annexin V-FITC/PI Apoptosis Assay
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The apoptosis analysis was performed by Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, China) according to the instructions of the manufacturer. In brief, the cell suspension was washed twice with precooled PBS and centrifuged at 1,000 × g for 5 min, followed by adding 100 μl of 1× binding buffer and blowing gently to make a single-cell suspension. Then 5 μl of Annexin V-FITC and 5 μl of PI staining solution were added and incubated for 10 min. Finally, 400 μl of 1× binding buffer was added and mixed gently. The images were captured with a fluorescent phase-contrast microscope (Nikon).
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