Protease inhibitor mixture solution and ethylenediaminetetraacetic acid (EDTA) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Phenylmethylsulfonyl fluoride (PMSF) was purchased from Sigma Aldrich Co., Ltd. (St. Louis, MO, USA). 2′,7’-Dichlorofluorescein diacetate (DCFH-DA) was obtained from Molecular Probes (Eugene, OR, USA). ECL Western Blotting Detection Reagents and pure nitrocellulose membranes were supplied by GE Healthcare (Piscataway, NJ, USA). Rabbit polyclonal antibodies against Nrf2, HO-1, p-p38, p-ERK1/2, NF-κBp65, and mouse monoclonal antibodies against, and p-IκBα, COX-2, iNOS, TNF-α, IL-6, histone, and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit anti-goat, goat anti-rabbit, and goat anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)-conjugated secondary antibodies were acquired from Santa Cruz Biotechnology, Inc. All other chemicals and reagents were purchased from Sigma Aldrich Co., Ltd. (St. Louis, MO, USA).
Pure nitrocellulose membranes
Manufactured by GE Healthcare
Sourced in United States
Pure nitrocellulose membranes are a type of laboratory equipment used for various applications, such as protein and nucleic acid detection and analysis. These membranes are made from purified nitrocellulose and provide a reliable and efficient platform for various blotting techniques.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor mixture solution, by Fujifilm (2 mentions)
Phenylmethylsulfonyl fluoride pmsf, by Merck Group (2 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
Ecl western blotting detection reagent, by GE Healthcare (1 mentions)
Tnf α, by Santa Cruz Biotechnology (1 mentions)
Nf κb p65, by Santa Cruz Biotechnology (1 mentions)
P erk1 2, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Ecl reagent, by Advansta (1 mentions)
Goat antirabbit and goat anti mouse igg horseradish peroxidase hrp conjugated secondary antibodies, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Enhanced chemiluminescence ecl western blotting detection reagent, by GE Healthcare (1 mentions)
3 protocols using pure nitrocellulose membranes
1
Antioxidant and Anti-Inflammatory Pathway Evaluation
2
ASFV Protein Analysis by Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For western blotting analysis, p30 and CD2v proteins were analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto pure nitrocellulose membranes (GE, Boston, MA, USA), which was blocked with 1% BSA in TBST at room temperature for 2 h, and then incubated with ASFV-positive or -negative serum as primary antibody at 4 °C overnight. After incubating with HRP-conjugated anti-pig secondary antibodies for 1 h at room temperature, signals were visualised by ECL reagent (Advansta, San Jose, CA, USA).
3
Colorectal Carcinoma Cell Line RKO Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human colorectal carcinoma cell line, RKO, was obtained from American Type Culture Collection (Manassas, Virginia, USA). Minimum essential medium (MEM) was purchased from Gibco Laboratories (Paisley, UK). Fetal bovine serum (FBS) and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) were purchased from Sigma-Aldrich Chemical Company (Dorset, UK). Six-well flat-bottom tissue culture plates were purchased from Nunc Inc. (Hereford, UK). Phenylmethylsulfonyl fluoride (PMSF) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA) and protease inhibitor mixture solution was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Enhanced chemiluminescence (ECL) western blotting detection reagents and pure nitrocellulose MEMbranes were purchased from GE Healthcare (Buckinghamshire, UK). Primary antibodies for the detection of caspase 3 and β-actin and goat antirabbit and goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). All chemicals and reagents were of analytical grade and purchased from Sigma-Aldrich Co., Ltd. unless otherwise specified.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!