Mouse anti myc epitope tag 9b11

Rabbit and rat antibodies to VAPB and PTPIP51 were as described and used at 1:200 for PLAs [10 (link)]. Mouse anti-VDAC1 was from Abcam (ab14734, RRID:AB 443,084) and rabbit anti-IP3 receptor type-1 was from Synaptic Systems (117,003, RRID:AB 2,619,787) and were used for PLAs at 1:200 and 1:100 respectively. Chicken anti-β-Tubulin Isotype III antibody was from Millipore (AB9354, RRID:AB 570,918) and used at 1:500 for immunostaining. Mouse anti-HA epitope-tag (6F2) and mouse anti-Myc epitope tag (9B11) antibodies were from Cell Signaling (H9658, RRID:AB 260,092; 2276 RRID:AB 331,783) and used at 1:500 for immunostaining and 1:1000 for immunoblots. Chicken anti-microtubule-associated protein-2 (MAP2) was from Genetex (GTX82661, RRID:AB 11,172,558) and used for immunostaining at 1:500. Rabbit anti-EGFP was from Invitrogen (A-11,122, RRID:AB 221,569) and used at 1:500. Rabbit anti-serine-9 phosphorylated GSK3β was from Cell Signaling (5558, RRID:AB 10,013,750) and used at 1:100. Species specific goat and donkey anti-mouse, anti-rabbit and anti-chicken Igs coupled to AlexaFluor-488, − 555, -594 or -647 were from Invitrogen and Jackson ImmunoResearch Labs.

Rabbit and rat antibodies to VAPB and PTPIP51, respectively, were as previously described (De Vos et al., 2012 (link)). Chicken anti‐microtubule‐associated protein‐2 (MAP2) was from Gentex. Rabbit anti‐IP3 receptor type 1 antibodies were from Synaptic Systems and Millipore (for PLAs in mice and neuronal cultures respectively), and from Cell Signaling (for immunoblots). Mouse anti‐MCU (CL3576), mouse anti‐VDAC (20B12AF2) and rabbit anti‐GFP were from Abcam. Mouse anti‐PDI (RL77) was from Thermo Fisher Scientific. Rabbit anti‐TOM20, goat anti‐VDAC1, mouse anti‐GFP (B2) and mouse anti‐total GSK3β (E‐11) were from Santa Cruz Biotechnology. Rabbit anti‐PTPIP51 was from Atlas. Mouse anti‐β‐Tubulin Isotype III and anti β‐actin were from Sigma. Rabbit anti‐serine‐9 phospho‐GSK3β (D85E12), mouse anti‐hemagglutin (HA) epitope tag (6E2) and mouse anti‐myc epitope tag (9B11) were from Cell Signaling. Mouse anti‐nucleophosmin antibody (B23/NPM1) was from ProteinTech. Species‐specific goat and donkey anti‐mouse, anti‐rabbit and anti‐chicken Igs coupled to AlexaFluor‐488, AlexaFluor‐594 or AlexaFluor‐647 were from Invitrogen or Jackson ImmunoResearch. Oxotremorine‐M (OxoM) was from Tocris.