At least 1 week after the surgery for implantation of EEG/EMG electrodes, mice were conditioned to handling, simulating drug injection and tethering in the recording setup for 5 d. On the day of the experiment, mice received an injection of VEH or CNO and EEG/EMG signals were recorded for 6 h. The injection and recording start time was 10:00 A.M. (ZT04), when homeostatic sleep pressure is high, an optimal time to study the wakefulness-promoting effect of the targeted neurons. Monopolar EEG and bipolar EMG signals were amplified (×1000) and digitized (sampling rate = 1024 Hz), respectively, with a model 1700 AC amplifier (A-M Systems) and a Micro3 1401 acquisition unit and Spike2 software (Cambridge Electronic Design); notch filters were used for each mouse recording (both after VEH and CNO) when 60 Hz electrical noise was present. All signals were bandpass filtered between 0.1 and 500 Hz (EEG) and between 10 and 500 Hz (EMG). Mouse behaviors were video recorded, in synchrony with the electrophysiologic recordings in Spike2.
Model 1700 ac amplifier
Manufactured by A-M Systems
Sourced in United States
The Model 1700 AC amplifier is a precision instrument designed to amplify and condition electrical signals. It features high input impedance, low noise, and accurate signal amplification. The core function of the Model 1700 is to provide reliable and consistent signal amplification for a variety of applications.
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Lab products found in correlation
2 protocols using model 1700 ac amplifier
1
Mouse EEG/EMG Recording for Wakefulness
2
EEG/EMG Monitoring of Chemogenetic Modulation
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At least 1 week after the surgery for implantation of EEG/EMG electrodes, mice were conditioned to handling, simulating drug injections and tethering in the recording setup for 5 days. On the day of the experiment, mice received an injection of vehicle or CNO and EEG/EMG signals were recorded for 6 hours (injection and recording start time: 10:00 AM). Monopolar EEG and bipolar EMG signals were amplified (X 1000) and digitized (sampling rate = 1024 Hz), respectively, with a Model 1700 AC amplifier (A-M Systems, Sequim, WA, USA) and a Micro3 1401 acquisition unit and Spike2 software (Cambridge Electronic Design, Cambridge, UK); notch filters were used for each mouse recording (both post-vehicle and CNO) when 60 Hz electrical noise was present. All signals were bandpass filtered between 0.1 -500 Hz (EEG) and 10 -500 Hz (EMG). Mouse behaviors were video recorded, in synchrony with the electrophysiologic recordings in Spike2.
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