The following materials and chemicals were used in the current experiment: A549 cells obtained from the cell bank of typical culture collection of the Chinese Academy of Sciences (Shanghai, China), dexamethasone-17-acetate (DEX, > 98%, Aladdin Co. Ltd), lipopolysaccharide (LPS) (Sigma-Aldrich), Roswell Park Memorial Institute-RPMI 1640 (Life Technology Cooperation's, USA), Dulbecco's modified Eagle medium (DMEM, Gibco, USA), 2,2-diphenyl-1-picrylhydrazyl-DPPH (Aladdin Chemistry Co., Ltd., China), trichloromethane (CHCl3, grade, analytical reagent (AR), methanol (grade, AR), acetone (grade, AR) and dimethyl sulfoxide (DMSO, grade, AR) (Sinopharm Chemical Reagent Company, China), enzyme-linked immunosorbent assay (ELISA) kit for TNF-α, IL-1β and IL-6 (BosterBio, Wuhan, China), ELISA kit for IL-4 and IL-10 (Elabscience Biotechnology), Lactate dehydrogenase (LDH) cytotoxicity assay kit (Beyotime, Biotechnology, China), TUNEL BrightGreen Apoptosis Detection Kit (A112-02, Vazyme, Nanjing, China), and 4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) (Sigma-Aldrich).
4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Merck Group
Sourced in China, United States
4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay to assess cell metabolic activity. It is a yellow tetrazolium dye that can be reduced to purple formazan crystals by cellular enzymes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Lactate dehydrogenase ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Tunel brightgreen apoptosis detection kit, by Vazyme (1 mentions)
Annexin 5 dead cell kit, by Merck Group (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Angiogenesis assay kit, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Welgene (1 mentions)
Huvecs, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Ags human gastric adenocarcinoma cells, by American Type Culture Collection (1 mentions)
2 protocols using 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt
1
Anti-inflammatory Effects of DEX in A549 Cells
2
Cytotoxicity and Apoptosis Assay in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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AGS human gastric adenocarcinoma cells and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (Manassas, VA, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum were purchased from WelGene (Daegu, South Korea). The cells were cultured in DMEM containing 10% fetal bovine serum, 100 U/ml penicillin, and 100 µg/ml streptomycin and were maintained in a 5% CO 2 incubator at 37°C. Antibodies against PTEN and p53 were purchased from Millipore, and beta-actin was purchased from Bethyl Laboratories, Inc. (Montgomery, TX, USA). Horseradish peroxidase-conjugated secondary antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). (4,5-Dimethylthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA). The angiogenesis assay kit, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit, and Annexin V/Dead Cell kit were purchased from Millipore (Darmstadt, Germany). The WST-1 kit was purchased from Enzo Life Sciences (Farmingdale, NY, USA).
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