Four-week-old plant leaves were infiltrated with sterile water (mock) or different Pst strains at OD600=0.02, and samples were collected at 0.5, 3, 6, 8h after infiltration. Three to four leaves from different plants were collected as one sample. Protein was extracted using Plasma Membrane Protein Isolation Kit (Invent) according to the manufacturer’s protocol. Concentration of the cytosolic protein was determined with Bradford protein assay kit (Bio-Rad). An equal amount of protein was loaded onto SDS-PAGE gel for western blot. BAK1 and RBOHD are detected in the immunoblot of total membrane fraction and other proteins are detected in the immunoblot of total protein extracts. Different PTI components were detected by following antibodies with indicated dilution: anti-RBOHD (Agrisera), 1:1000; anti-BAK1 (Agrisera), 1:5000; anti-BIK1 (Agrisera), 1:3000; anti-MPK3 (Sigma-Aldrich), 1:2500; anti-MPK6 (Sigma-Aldrich), 1:5000. Goat Anti-Rabbit lgG HRP (Abmart; 1:5000) was used as secondary antibody. The protein image was taken using the Tanon-5200 imaging system (Tanon).
Anti bak1
Manufactured by Agrisera
Anti-BAK1 is a laboratory product that recognizes and binds to the BAK1 protein. BAK1 is a key regulator of plant cell growth and development. The Anti-BAK1 product can be used for detection and analysis of the BAK1 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti bik1, by Agrisera (2 mentions)
Plasma membrane protein isolation kit, by Invent Biotechnologies (2 mentions)
Anti rbohd, by Agrisera (2 mentions)
Anti mpk6, by Merck Group (2 mentions)
Anti mpk3, by Merck Group (2 mentions)
Bradford protein assay kit, by Bio-Rad (2 mentions)
Goat anti rabbit lgg hrp, by Abmart (1 mentions)
Anti rabbit, by Merck Group (1 mentions)
Anti v5, by Merck Group (1 mentions)
Anti goat, by Merck Group (1 mentions)
Anti mouse, by Merck Group (1 mentions)
Anti atpase, by Agrisera (1 mentions)
Anti vp16, by Santa Cruz Biotechnology (1 mentions)
Anti luciferase, by Merck Group (1 mentions)
Amersham imager 600, by Cytiva (1 mentions)
Ecl western blotting detection system, by GE Healthcare (1 mentions)
Anti ha, by Merck Group (1 mentions)
Anti gfp, by Abcam (1 mentions)
3 protocols using anti bak1
1
PTI Components Detection Protocol
2
Western Blot Immunodetection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteins were separated, blotted, and incubated with antibodies as described by Schulze et al. (2010) but using 8% SDS gels and the following antibody dilutions: anti-GFP (Abcam), 1:3,000; anti-HA (Sigma), 1:2,000; anti-V5 (Sigma), 1:2,000; anti-BAK1 (Agrisera), 1:3,000; anti-BIR3 antibodies were obtained from rabbits immunized with the peptide CVGSRDSNDSSFNN fused to KLH (Agrisera) 1:500; anti-luciferase (Sigma), 1:3,000; anti-VP16 (Santa Cruz), 1:1000; anti-ATPase (Agrisera), 1:5,000; 1:5,000; anti-rabbit (Sigma),1:50,000; anti-goat (Sigma), 1:10,000; anti-mouse (Sigma), 1:10,000.
Chemiluminescence was detected with the ECL Western blotting detection system (GE Healthcare) and a CCD camera (Amersham Imager 600). If figures were reconstituted from images of blots, lanes from the same blot are shown in one panel of a figure even if they were in a different order on the original blot (separated by dotted lines). Data from different blots are shown in separate figure parts.
Chemiluminescence was detected with the ECL Western blotting detection system (GE Healthcare) and a CCD camera (Amersham Imager 600). If figures were reconstituted from images of blots, lanes from the same blot are shown in one panel of a figure even if they were in a different order on the original blot (separated by dotted lines). Data from different blots are shown in separate figure parts.
3
Monitoring Immune Responses in Plants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four-week-old plant leaves were infiltrated with sterile water (mock) or different Pst strains at OD600=0.02, and samples were collected at 0.5, 3, 6, 8h after infiltration. Three to four leaves from different plants were collected as one sample. Protein was extracted using Plasma Membrane Protein Isolation Kit (Invent) according to the manufacturer's protocol. Concentration of the cytosolic protein was determined with Bradford protein assay kit (Bio-Rad). An equal amount of protein was loaded onto SDS-PAGE gel for western blot. Different PTI components were detected by following antibodies with indicated dilution: anti-RBOHD (Agrisera), 1:1000; anti-BAK1 (Agrisera), 1:5000; anti-BIK1 (Agrisera), 1:3000; anti-MPK3 (Sigma-Aldrich), 1:2500; anti-MPK6 (Sigma-Aldrich), 1:5000.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!