Sca 1

FAPs were isolated from C57Bl6 mdx mice at the end of the treatments immediately after the sacrifice. Hind limb muscles for each mouse were minced and put into a 15 ml tube containing 4 ml of digest solution in HBSS (#24020-091,GIBCO) containing 2 mg/ml Collagenase A (#10103586001, Roche), 2.4 U/ml Dispase II (#04942078001, Roche), 10 ng/ml DNase I (#11284932001, Roche) for 90 min at 37°C. Cells were filtered through 100um, 70um and 40um cell strainers (#08-771-19, #08-771-2, #08-771-1, BD Falcon) and resuspended in 0.5 ml of HBSS containing 0.2% w/v BSA and 1% v/v Penicillin-Streptomycin for the staining of cell surface antigens 30 min on ice. The following antibodies were used: CD45-eFluor 450 (1:50, #48-0451-82, eBiosciences), CD31-eFluor 450 (1:50, #48-0311-82, eBioscience), Ter119-eFluor 450 (1:50, #48-5921-82, eBiosciences), Itga7-649 (1:500, #67-0010-01, AbLab) and Sca1-FITC (1:50, 5981-82, eBioscience). Cells were finally washed and resuspended in 1 ml of HBSS containing 0.2% w/v BSA and 1% v/v Penicillin-Streptomycin. FAPs were isolated as Ter119 -/CD45 -/CD31 -/ Itga7 -/Sca1 + cells using a Beckman Coulter MoFlo Legacy high-speed cell sorter.