Rabbit specific elisa kits

Concentrations of cytokines and oxidative modification products were determined in 10 % (weight/volume) lung homogenate prepared using 0.1 M phosphate buffer (PBS, pH 7.4) by Polytron homogenizer PT 1200 E (Kinematica AG, Switzerland). Oxidative damage to proteins was determined using OxiSelect TM Nitrotyrosine ELISA Kit (Cell Biolabs, Inc., USA) and expressed as concentration of 3-nitrotyrosine in nanomoles (nM 3NT). Oxidative damage to lipids expressed as concentration of thiobarbituric acid reacting substances (TBARS) was determined by OxiSelect TM TBARS Assay Kit (Cell Biolabs Inc., USA), and was shown as concentration of malondialdehyde in micromoles (µM MDA). To evaluate the changes in oxidative status in plasma, concentrations of 3NT and MDA were detected in initial (P1) and final (PF) plasma and expressed as a ratio PF/P1. Concentrations of IL-2, IL-6, IL-13 and TNF-α were quantified in duplicate using rabbit-specific ELISA kits (USCN Life Science Inc., China) according to the manufacturer's instructions and expressed in pg/ml.

Oxidative damage to proteins was determined using OxiSelectTM Nitrotyrosine ELISA Kit (Cell Biolabs Inc., USA) and expressed as a concentration of 3-nitrotyrosine in nanomoles (nM 3NT). Oxidative damage to lipids expressed as concentration of thiobarbituric acid reacting substances (TBARS) was determined by OxiSelectTM TBARS Assay Kit (Cell Biolabs Inc., USA), and was shown as a concentration of malondialdehyde in micromoles (µM MDA). Concentrations of TNFα, IL-6, IL-8 were quantified in duplicate using rabbit-specific ELISA kits (USCN Life Science Inc., China) according to the manufacturer's instructions and expressed in pg/ml.