Moisture, lipid, protein, and ash contents were determined on raw almonds by AOAC methods (2010). Total carbohydrates were calculated by difference.
Fatty acid composition was determined on almond oil. Fatty acid methyl esters were prepared from almond oil by transmethylation with a solution of 30 g/L sulfuric acid in methanol. The fatty acid methyl esters of total lipids were analyzed on a Perkin Elmer Clarus 600 gas-liquid chromatograph (Waltham, Mass., U.S.A.) equipped with a flame ionization detector (FID). A SAC TM -5 capillary column (30 m × 0.25 mm i.d., 0.25-μm film thickness; C#24156, Supelco, Bellefonte, Pa., U.S.A.) was used. Separation, identification, and quantification of the fatty acid methyl esters were performed according to Riveros and others 2010.
Fatty acid composition was determined on almond oil. Fatty acid methyl esters were prepared from almond oil by transmethylation with a solution of 30 g/L sulfuric acid in methanol. The fatty acid methyl esters of total lipids were analyzed on a Perkin Elmer Clarus 600 gas-liquid chromatograph (Waltham, Mass., U.S.A.) equipped with a flame ionization detector (FID). A SAC TM -5 capillary column (30 m × 0.25 mm i.d., 0.25-μm film thickness; C#24156, Supelco, Bellefonte, Pa., U.S.A.) was used. Separation, identification, and quantification of the fatty acid methyl esters were performed according to Riveros and others 2010.