All chemicals were used as supplied by vendors without further purification. Imidazole, Invitrogen Novex WedgeWell 14% Tris-Glycine Mini Protein Gels, Isopropy-ß-D-thiogalactopyranoside (IPTG), lysozyme, NativePAGE 4 to 16% Bis-Tris Mini Protein Gels, NativeMark Unstained Protein Standard, Spectra Multicolor Broad Range Protein Ladder, Thermo Scientific Pierce 660 nm Protein Assay Reagent, Tris base, Tris HCl, all restriction enzymes, and all cell culture media and reagents were purchased from Fisher Scientific, Inc. (USA). Gibson Assembly Master Mix was purchased from NEB (USA). Amicon Ultra 0.5 mL centrifugal units and Benzonase nuclease were purchased from MilliporeSigma (USA). BL21 (DE3) Electrocompetent Cells used for Escherichia coli expression were also purchased from MilliporeSigma (USA). Bis-tris propane from Research Products International (USA) was used for the assembly buffer. Ni-NTA agarose from Gold Biotechnology, Inc. (USA) was used for His-tagged protein purification.
Amicon ultra 0.5 ml centrifugal units
Manufactured by Merck Group
Sourced in United States
The Amicon Ultra 0.5 mL centrifugal units are a type of laboratory equipment used for the concentration and purification of samples. They feature a semi-permeable membrane that allows the passage of small molecules while retaining larger molecules, such as proteins or macromolecules, during the centrifugation process.
Automatically generated - may contain errors
Lab products found in correlation
Tris base, by Thermo Fisher Scientific (2 mentions)
Gibson assembly master mix, by New England Biolabs (2 mentions)
Novex wedgewell 14 tris glycine mini protein gels, by Thermo Fisher Scientific (2 mentions)
Benzonase nuclease, by Merck Group (2 mentions)
Lysozyme, by Thermo Fisher Scientific (1 mentions)
Nativemark unstained protein standard, by Thermo Fisher Scientific (1 mentions)
Spectra multicolor broad range protein ladder, by Thermo Fisher Scientific (1 mentions)
Ni nta agarose, by GoldBio (1 mentions)
Pierce 660 nm protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Nativepage 4 to 16 bis tris mini protein gels, by Thermo Fisher Scientific (1 mentions)
Tris hcl, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Thermo Fisher Scientific (1 mentions)
Q5 high fidelity dna polymerase, by New England Biolabs (1 mentions)
2 protocols using amicon ultra 0.5 ml centrifugal units
1
Optimized His-tagged Protein Purification
2
Protein Expression and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were used as supplied by vendors without further purification. Imidazole, Invitrogen Novex WedgeWell 14% tris-glycine Mini Protein Gels, NativePAGE 4 to 16% bis-tris Mini Protein Gels, NativeMark Unstained Protein Standard, Isopropy-b-d -thiogalactopyranoside (IPTG), lysozyme, Spectra Multicolor Broad Range Protein Ladder, Pierce BCA protein assay kit, Tris base, Tris HCl, all restriction enzymes, and all cell culture media and reagents were purchased from Fisher Scientific, Inc. (USA). Gibson Assembly Master Mix and Q5 High-Fidelity DNA Polymerase were purchased from NEB (USA). Amicon Ultra-0.5 mL centrifugal units, Benzonase nuclease, and BL21 (DE3) Electrocompetent Cells used for E. coli expression were purchased from MilliporeSigma (USA). Ni-NTA agarose from Gold Biotechnology, Inc. (USA) was used for His-tagged protein purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!