1 myristoyl 2 stearoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine is a phospholipid compound. It serves as a fundamental structural component in biological membranes.

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Lab products found in correlation

Triethanolamine teoa, by Merck Group (1 mentions) Copper 2 gluconate, by Merck Group (1 mentions) Gemcitabine hydrochloride, by LC Laboratories (1 mentions) Exosome depleted fbs, by Thermo Fisher Scientific (1 mentions) Fluorescein disodium, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 1 2 distearoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Lipopolysaccharide lps, by InvivoGen (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Mda mb 231 breast cancer cells, by American Type Culture Collection (1 mentions) Cardiolipin, by Avanti Polar Lipids (1 mentions) Bicinchoninic acid protein kit, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt assay kit, by Merck Group (1 mentions) Topfluor cl, by Avanti Polar Lipids (1 mentions) Whatman, by Cytiva (1 mentions)

3 protocols using 1 myristoyl 2 stearoyl sn glycero 3 phosphocholine

Lipid-based Nanocarrier Formulations

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1-Palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine
(Lyso-PPC), 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine
(Lyso-SPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC),
1,2-disteraroyl-sn-glycero-3-phosphocholine (DSPC),
1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC),
1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine (MSPC),
and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy
polyetheneglycol-2000 (DSPE-PEG2k) were purchased from Avanti Polar Lipids Inc.
(Alabaster, AL). Triethanolamine (TEOA) and copper (II) gluconate were purchased
from Sigma (St. Louis, MO). Gemcitabine hydrochloride was purchased from LC
Laboratories (Woburn, MA).
KPC (mT4) cells were a gift from Dr. David Tuveson (Cold Spring Harbor
Laboratory, Cold Spring Harbor, NY). KPC cells were cultured in
Dulbecco’s Modified Eagle medium (DMEM) containing 4500 mg/L glucose,
L-glutamine and sodium pyruvate (# 11995, Invitrogen, San Diego, CA) and
supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin.
The neu deletion (NDL) metastatic mammary carcinoma cell line
was a gift from Dr. Alexander Borowsky (University of California- Davis, Davis,
CA). NDL cells were cultured in high-glucose DMEM, supplemented with 10% fetal
bovine serum, 1% penicillin-streptomycin, 0.584 g/L L-glutamine and 0.110 g/L
sodium pyruvate. All cells were maintained at 37°C in a humidified 5%
CO₂ incubator.

Tucci S.T., Kheirolomoom A., Ingham E.S., Mahakian L.M., Tam S.M., Foiret J., Hubbard N.E., Borowsky A.D., Baikoghli M., Cheng R.H, & Ferrara K.W. (2019). Tumor-specific delivery of gemcitabine with activatable liposomes. Journal of controlled release : official journal of the Controlled Release Society, 309, 277-288.

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Lipid Characterization in Macrophage Activation

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1,2-dipalmitoyl-sn-glycero-3-phospocholine (DPPC), 1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine (MSPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were purchased from Avanti Polar Lipids Inc. (Birmingham, AL, USA). Lissamine Rhodamine B 1,2-dihexadecanoyl-sn-glycero-3 phosphoethanolamine (rhodamine-DHPE) was provided by ThermoFisher Scientific Co., Ltd. (USA). Disodium fluorescein was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Lipopolysaccharide (LPS) was provided by InvivoGen (San Diego, CA, USA), and Interferon gamma (INF-γ) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The cell line J774A.1 was obtained from American Type Culture Collection (ATCC, USA). Fetal bovine serum (FBS) and exosome-depleted FBS were provided by Thermo Scientific (Paisley, UK). All the other reagents used in the experiments were of analytical grade (> 98%).

Barone A., Zimbo A.M., d’Avanzo N., Tolomeo A.M., Ruga S., Cardamone A., Celia C., Scalise M., Torella D., La Deda M., Iaccino E, & Paolino D. (2023). Thermoresponsive M1 macrophage-derived hybrid nanovesicles for improved in vivo tumor targeting. Drug Delivery and Translational Research, 13(12), 3154-3168.

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Lipid-based Nanoparticle Formulation for Cancer

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-myristoyl-2-stearoyl-sn-glycero-3-phosphocholine (MSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG (2000)), cardiolipin (CL), 1,1′,2,2′-tetraoleoyl cardiolipin [4-(dipyrrometheneboron difluoride)butanoyl] (ammonium salt), Distearoylphosphatidylcholine (DSPC), TopFluor^® CL were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Cholesterol and ammonium sulfate were purchased from JT Baker (Phillipsburg, NJ, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM) and other reagents for cell culture were purchased from Mediatech (Manassas, VA, USA). Daunorubicin was purchased from AvaChem Scientific (San Antonio, TX, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay kit and Phosphate Buffered Saline (PBS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). DAPI (4′,6-diamidino-2-phenylindole) and Bicinchoninic acid protein kit was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Polycarbonate membrane (0.08 μm) was purchased from Whatman plc (Maidstone, Kent, UK). MDA-MB-231 breast cancer cells were obtained from American Type Culture Collection (Manassas, VA, USA).

Alrbyawi H., Boddu S.H., Poudel I., Annaji M., Mita N., Arnold R.D., Tiwari A.K, & Babu R.J. (2022). Cardiolipin for Enhanced Cellular Uptake and Cytotoxicity of Thermosensitive Liposome-Encapsulated Daunorubicin toward Breast Cancer Cell Lines. International Journal of Molecular Sciences, 23(19), 11763.

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