Apoptosis was determined by flow cytometry using an Annexin V-FITC/PI dual staining kit (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China). Cells incubated with 2 μM GA for the indicated times, were collected, washed and stained in working solution (500 μl binding buffer with 5 μl Annexin V-FITC and 5 μl propidium iodide) for 15 min at room temperature in the dark. Cells were then washed and resuspended with binding buffer. Apoptotic cells were determined by BD FACSCanto II flow cytometry. Annexin V-FITC-positive and Annexin V-FITC-plus-PI positive cells were determined as apoptotic cells.
Annexin 5 fitc pi dual staining kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V-FITC/PI dual staining kit is a laboratory tool used to detect and quantify apoptotic cells. It combines two fluorescent dyes, Annexin V-FITC and Propidium Iodide (PI), to differentiate between early apoptotic, late apoptotic, and necrotic cells in a sample. The kit provides a standardized method for this analysis.
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3 protocols using annexin 5 fitc pi dual staining kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Apoptosis Quantification by Flow Cytometry
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Cell apoptosis analysis was estimated using an Annexin V-FITC/PI dual staining kit (Nanjing KeyGen Biotech Co., Ltd.). Briefly, cells were cultured in 6-well plates and incubated with pyrimethamine for 96 h. Then, the cells were collected and suspended in binding buffer with PI and Annexin V-FITC at RT for 15 min. Cell apoptosis rates were finally detected by flow cytometry. Annexin V-FITC+ and annexin V-FITC+-PI+ cells were considered apoptotic cells.
3
Quantifying Cell Apoptosis via Annexin V-FITC/PI
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An annexin V-FITC/PI dual staining kit (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) was used to detect cell apoptosis. Cells were treated with or without TSN for the indicated times, after which the cells were collected, washed, and suspended in 500 µl of binding buffer, and mixed with 5 µl of annexin V-FITC and 5 µl of propidium iodide. The solutions were incubated in the dark at room temperature for 15 min before analysis. Cell apoptosis rates were measured using a BD FACSCanto II flow cytometer. Annexin V-FITC-positive and annexin V-FITC-plus PI-positive cells were reported as undergoing apoptosis.
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