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Trypsin edta

Manufactured by Vitrocell
Sourced in Brazil

Trypsin/EDTA is a laboratory solution used for the dissociation and detachment of adherent cells from cell culture surfaces. It contains the enzymes trypsin and EDTA, which work together to break down the proteins and cell-cell adhesions, allowing the cells to be harvested and subcultured.

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2 protocols using trypsin edta

1

Cytotoxicity Evaluation of Chemical Reagents

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Chromatography grade 2-propanol, acetonitrile, and methanol were obtained from Merck (Germany). The cytotoxicity assay kits were purchased from Xenometrix (Switzerland). The DNA extraction solutions were obtained from QIAGEN (Valencia, CA). The culture media, FCS, and trypsin/EDTA were acquired from Vitrocell – Embriolife (Brazil). All other chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO).
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2

Lipid Nanocarrier Formulation Components

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Sodium oleate and cholesterol were purchased from Sigma-Aldrich® (St. Louis, MO, USA). Soy phosphatidylcholine was purchased from Lucas Meyer Gmbh & Co (Hamburg, Germany). Phosphate buffer solution was prepared from sodium monohydrogen phosphate and dihydrogen phosphate that were purchased from Merck (Darmstadt, Germany). Polyoxyl-40 hydrogenated castor oil was purchased from Pharma Special (Itapevi, Sao Paulo, Brazil). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, gentamicin sulfate, amphotericin B, and trypsin/EDTA were purchased from Vitrocell Embriolife (Campinas, Sao Paulo, Brazil).
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