Pectin powder from citrus was purchased from Daejung Chemicals & Metals Co., Ltd. (Siheung, Korea). Glycerol was obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Korea). Calcium chloride (CaCl2) and ZnO nanopowder were purchased from Sigma Aldrich Chemicals (St. Louis, MO, USA). Nutrient broth (NB) was purchased from DB DifcoTM (Sparks, MD, USA). Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538 P were procured from the American Type Culture Collection (ATCC).
Staphylococcus aureus atcc 6538p
Manufactured by American Type Culture Collection
Sourced in United States
Staphylococcus aureus ATCC 6538P is a bacterial strain obtained from the American Type Culture Collection (ATCC). It is a Gram-positive, cocci-shaped bacterium commonly found as a commensal organism on human skin and mucous membranes. This strain is commonly used as a reference or quality control organism in microbiological testing and research.
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Lab products found in correlation
Calcium chloride cacl2, by Merck Group (1 mentions)
Zno nanopowder, by Merck Group (1 mentions)
Glycerol, by Duksan Pure Chemicals (1 mentions)
Escherichia coli atcc 8739, by American Type Culture Collection (1 mentions)
Maldi mass spectrometer, by Bruker (1 mentions)
Staphylococcus aureus atcc 25923, by American Type Culture Collection (1 mentions)
Enterococcus faecalis atcc 29212, by American Type Culture Collection (1 mentions)
Bacillus subtilis atcc 6633, by American Type Culture Collection (1 mentions)
Staphylococcus aureus atcc 43300, by American Type Culture Collection (1 mentions)
Escherichia coli atcc 35218, by American Type Culture Collection (1 mentions)
E faecalis atcc 51299, by American Type Culture Collection (1 mentions)
5 protocols using staphylococcus aureus atcc 6538p
1
Pectin-based antimicrobial nanocomposites
2
Biofilm-forming Reference Strains
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Uropathogenic Escherichia coli ATCC 700928 (CFT073) and Staphylococcus aureus ATCC 6538P were biofilm-forming reference strains obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). E. coli K-12 MG1655 was a weak biofilm producer strain. The microorganisms were grown in Brain Heart Infusion broth (BHI) (Oxoid) and stored in 15% glycerol-BHI at −80 °C.
3
Bacterial Strain Characterization and Storage
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Bacterial strains employed in this study were: Staphylococcus aureus ATCC6538P (American Type Culture Collection, Manassas, VA, USA), Escherichia coli ATCC13762, Enterococcus hirae ATCC10541 and Multi Drug Resistant (MDR) clinical isolates of Meticillin Resistant S. aureus (MRSA), S. epidermidis, Extended Spectrum Beta Lactamase (ESBL)-producing E. coli (2 clinical strains), MDR Acinetobacter baumannii complex (2 clinical strains), and Candida albicans. The identification of clinical isolates was performed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometer (Bruker Daltonics, Billerica, MA, USA) [38 (link)], and by biochemical phenotyping method in a VITEK®2 System (BioMérieux Italia S.p.a., Bagno A Ripoli (FI), Italy), according to the manufacturer’s instruction. The profile of susceptibility to antibiotics was also evaluated using the VITEK®2 System [39 (link),40 (link)]. Bacterial isolates were aerobically cultured at 37 °C in Tryptic Soy (TS) broth/agar medium (Oxoid, Oxoid S.p.a., Rodano, Milan, Italy). Bacterial strains were stored frozen at −80 °C in TS broth with 10% glycerin (v/v) (Carlo Erba, Reagents, Milan, Italy).
4
Bacterial Strain Cultivation and Storage
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Escherichia coli ATCC 35218, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P (MSSA), Staphylococcus aureus ATCC 25923 (MSSA), Staphylococcus aureus ATCC 43300 (MRSA), Enterococcus faecalis ATCC 29212, and E. faecalis ATCC 51299 (VanB phenotype) were obtained from the American Type Culture Collection (ATCC). Enterococcus faecalis 9160188401-EF-34 (VanA phenotype) and Staphylococcus epidermidis strain 4 are clinical isolates, kindly provided by Laboratorio Microbiologia Clinica – Ospedale di Circolo, Varese, Italy. Staphylococcus haemolyticus 3902 is a teicoplanin-resistant clinical isolate (Beltrametti et al., 2003 (link)), received from FIIRV (Fondazione Istituto Insubrico Ricerca per la Vita), Gerenzano Varese, Italy. For long-term preservation, bacterial cultures were stored at -80°C in 10% v/v glycerol.
E. coli and B. subtilis were routinely grown at 37°C with continuous shaking at 200rpm (revolutions per minute) in LB broth. S. aureus, S. epidermidis, S. haemolyticus, and E. faecalis strains were propagated in the same conditions in MHB2. For exponential growth, overnight cultures were diluted in fresh medium at an optical density at 600nm (OD600nm) of 0.1 and incubated as above.
E. coli and B. subtilis were routinely grown at 37°C with continuous shaking at 200rpm (revolutions per minute) in LB broth. S. aureus, S. epidermidis, S. haemolyticus, and E. faecalis strains were propagated in the same conditions in MHB2. For exponential growth, overnight cultures were diluted in fresh medium at an optical density at 600nm (OD600nm) of 0.1 and incubated as above.
5
Bacterial Culture Protocols for Research
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Escherichia coli ATCC 35218, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 10145, Salmonella enterica subsp. typhimurium ATCC 6994, and Staphylococcus aureus ATCC 6538P (methicillin susceptible S. aureus, MSSA) were obtained from the American Type Culture Collection (ATCC). Acinetobacter baumannii, Enterococcus faecalis, and Yersinia enterocolitica were clinical isolates. E. coli, B. subtilis, P. aeruginosa, S. enterica subsp. typhimurium, E. faecalis, Y. enterocolitica, and A. baumannii were propagated overnight in Luria Bertani medium (LB, 2% tryptone, 2% yeast extract, and 1% NaCl). S. aureus in Mueller Hinton broth 2 (MHB2, 0.3% beef infusion solids, 1.75% casein hydrolysate, and 0.15% starch) with continuous shaking at 200 rpm and 37°C. For exponential growth, overnight cultures were transferred to fresh medium: start cultures showed an optical density at 600 nm (OD600 nm) of 0.1. Storage at −20°C in 20% glycerol was used for long-term preservation.
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